Restriction map for
pF1KB7763
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AvrII BamHI BbrPI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DpnII DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FbaI
FseI HgaI HindIII KpnI KspI
MamI MboI MfeI MflI MluI
MroI MspA1I MunI MvnI NaeI
NdeI NdeII NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RcaI RsrII
SacI SacII SalI Sau3AI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 208 GTAGAC GT^[AC][GT]AC
AclI 69 AACGTT AA^CGTT
AcyI 85 GGCGCC G[GA]^CG[CT]C
AflII 1159 CTTAAG C^TTAAG
AocI 617 CCTCAGG CC^T[ACGT]AGG
AspI 1284 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 249 TGCGCA TGC^GCA
BalI 882 TGGCCA TGG^CCA
BanI 85 GGCGCC G^G[CT][GA]CC
BanII 1100 GAGCCC G[GA]GC[CT]^C
BbeI 85 GGCGCC GGCGC^C
BbsI 1020 GAAGAC GAAGAC(2/6)
BcgI 240 CGATGGACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 197 CCCGG CC^[GC]GG
BfrI 1159 CTTAAG C^TTAAG
BmyI 1100 GAGCCC G[AGT]GC[ACT]^C
BpuAI 1020 GAAGAC GAAGAC(2/6)
BsaHI 85 GGCGCC G[GA]^CG[CT]C
BseRI 952 GAGGAG GAGGAG(10/8)
BsgI 281 GTGCAG GTGCAG(16/14)
BsmI 291 GAATGC GAATGC(1/-1)
Bsp1286I 1100 GAGCCC G[AGT]GC[ACT]^C
BstAPI 685 GCAGCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 622 GGTGACC G^GT[ACGT]ACC
BstPI 622 GGTGACC G^GT[ACGT]ACC
Bsu36I 617 CCTCAGG CC^T[ACGT]AGG
DraII 214 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 253 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 882 TGGCCA [CT]^GGCC[GA]
Eco81I 617 CCTCAGG CC^T[ACGT]AGG
EcoNI 868 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 214 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 622 GGTGACC G^GT[ACGT]ACC
FspI 249 TGCGCA TGC^GCA
HapII 198 CCGG C^CGG
Hin1I 85 GGCGCC G[GA]^CG[CT]C
HpaI 574 GTTAAC GTT^AAC
HpaII 198 CCGG C^CGG
KasI 85 GGCGCC G^GCGCC
MluNI 882 TGGCCA TGG^CCA
MscI 882 TGGCCA TGG^CCA
MspI 198 CCGG C^CGG
NarI 85 GGCGCC GG^CGCC
NciI 197 CCCGG CC^[GC]GG
PflFI 1284 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 98 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 214 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 69 AACGTT AA^CGTT
PstI 233 CTGCAG CTGCA^G
SapI 1097 GAAGAGC GCTCTTC(1/4)
ScaI 165 AGTACT AGT^ACT
SfoI 85 GGCGCC GGC^GCC
SmlI 1159 CTTAAG C^T[CT][GA]AG
TfiI 483 GAATC G^A[AT]TC
Tth111I 1284 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 98 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 662 CCACCTCAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 215 GGACC G^G[AT]CC
1298 GGACC
BbvCI 694 GCTGAGG CCTCAGC(-5/-2)
1225 GCTGAGG
BfaI 31 CTAG C^TAG
1316 CTAG
BsmAI 955 GAGAC GTCTC(1/5)
1273 GAGAC
BspMI 933 ACCTGC ACCTGC(4/8)
178 GCAGGT
BsrDI 475 GCAATG GCAATG(2/0)
1052 GCAATG
BstF5I 462 CATCC GGATG(2/0)
192 GGATG
DsaI 1208 CCATGG C^C[GA][CT]GG
1325 CCATGG
FokI 462 CATCC GGATG(9/13)
192 GGATG
HaeII 85 GGCGCC [GA]GCGC^[CT]
338 AGCGCC
HincII 574 GTTAAC GT[CT]^[GA]AC
769 GTTGAC
HindII 574 GTTAAC GT[CT]^[GA]AC
769 GTTGAC
MaeI 31 CTAG C^TAG
1316 CTAG
MaeII 70 ACGT A^CGT
559 ACGT
NcoI 1208 CCATGG C^CATGG
1325 CCATGG
NspI 246 ACATGC [GA]CATG^[CT]
302 ACATGC
PleI 772 GACTC GAGTC(4/5)
836 GAGTC
TspRI 668 CAGTG CA[GC]TG[ACGT][ACGT]^
1029 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BpmI BsaJI BsiYI BslI BsmFI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII EcoT14I
Fnu4HI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MslI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI SfcI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TthHB8I