Restriction map for
pF1KB8991
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeII MaeIII MamI MflI MluI
MseI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp45I
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 459 TCCGGA T^CCGGA
AcsI 35 GAATTT [GA]^AATT[CT]
ApaI 3 GGGCCC GGGCC^C
ApoI 35 GAATTT [GA]^AATT[CT]
AspHI 123 GAGCAC G[AT]GC[AT]^C
BalI 302 TGGCCA TGG^CCA
BcgI 227 CGAGGCGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 356 CTAG C^TAG
BglI 295 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 459 TCCGGA [AT]^CCGG[AT]
BseAI 459 TCCGGA T^CCGGA
BsiHKAI 123 GAGCAC G[AT]GC[AT]^C
BspEI 459 TCCGGA T^CCGGA
BsrBI 68 GAGCGG CCGCTC(-3/-3)
BstF5I 132 CATCC GGATG(2/0)
DdeI 175 CTCAG C^T[ACGT]AG
DpnII 222 GATC ^GATC
EaeI 302 TGGCCA [CT]^GGCC[GA]
EcoT22I 331 ATGCAT ATGCA^T
FokI 132 CATCC GGATG(9/13)
HgaI 189 GACGC GACGC(5/10)
MaeI 356 CTAG C^TAG
MboI 222 GATC ^GATC
MboII 107 TCTTC GAAGA(8/7)
MfeI 391 CAATTG C^AATTG
MluNI 302 TGGCCA TGG^CCA
MroI 459 TCCGGA T^CCGGA
MscI 302 TGGCCA TGG^CCA
MslI 378 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 391 CAATTG C^AATTG
NdeII 222 GATC ^GATC
NlaIII 378 CATG CATG^
NsiI 331 ATGCAT ATGCA^T
PaeR7I 225 CTCGAG C^TCGAG
PflMI 345 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 170 AGGACCT [GA]G^G[AT]CC[CT]
PstI 24 CTGCAG CTGCA^G
PvuII 349 CAGCTG CAG^CTG
Sau3AI 222 GATC ^GATC
SfaNI 448 GATGC GCATC(5/9)
SfcI 24 CTGCAG C^T[GA][CT]AG
SfiI 294 GGCCACCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 7 CCCGGG CCC^GGG
SmlI 225 CTCGAG C^T[CT][GA]AG
TaqI 226 TCGA T^CGA
TfiI 456 GAATC G^A[AT]TC
TspRI 202 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 226 TCGA T^CGA
Van91I 345 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 155 CCAGCCCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 225 CTCGAG C^TCGAG
XmaCI 7 CCCGGG C^CCGGG
XmaI 7 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaI 7 CCCGGG C^[CT]CG[GA]G
225 CTCGAG
AvaII 171 GGACC G^G[AT]CC
369 GGACC
BanII 3 GGGCCC G[GA]GC[CT]^C
46 GGGCTC
BcnI 7 CCCGG CC^[GC]GG
8 CCGGG
BpmI 49 CTCCAG CTGGAG(16/14)
166 CTGGAG
BseRI 139 GAGGAG GAGGAG(10/8)
268 GAGGAG
BsoBI 7 CCCGGG C^[CT]CG[GA]G
225 CTCGAG
BssHII 192 GCGCGC G^CGCGC
216 GCGCGC
HapII 8 CCGG C^CGG
460 CCGG
HpaII 8 CCGG C^CGG
460 CCGG
MspI 8 CCGG C^CGG
460 CCGG
NciI 7 CCCGG CC^[GC]GG
8 CCGGG
PleI 56 GACTC GAGTC(4/5)
427 GACTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HhaI
HinP1I HinfI ItaI MnlI MspA1I
MvaI MvnI MwoI NlaIV Sau96I
ScrFI TseI Tsp509I