Restriction map for
pF1KB7337
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccIII AclI AcyI AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI AspI AviII AvrII
BbeI BbrPI BcnI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HapII HgaI Hin1I
HpaI HpaII KasI KspI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 3102 GTCGAC GT^[AC][GT]AC
AccII 158 CGCG CG^CG
AflII 1111 CTTAAG C^TTAAG
AhdI 127 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1442 GGGCCC GGGCC^C
Asp700I 1054 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 127 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3124 CTCGGG C^[CT]CG[GA]G
BamHI 657 GGATCC G^GATCC
BbvCI 2841 CCTCAGC CCTCAGC(-5/-2)
BcgI 870 CGATTTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1079 TGATCA T^GATCA
BfrI 1111 CTTAAG C^TTAAG
BsaI 2233 GGTCTC GGTCTC(1/5)
BsmBI 2723 GAGACG CGTCTC(1/5)
BsoBI 3124 CTCGGG C^[CT]CG[GA]G
BspHI 1184 TCATGA T^CATGA
BssSI 1927 CACGAG CACGAG(-5/-1)
BstAPI 738 GCAGGCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2770 GGTAACC G^GT[ACGT]ACC
BstPI 2770 GGTAACC G^GT[ACGT]ACC
BstUI 158 CGCG CG^CG
CfoI 648 GCGC GCG^C
DraI 1773 TTTAAA TTT^AAA
DrdI 2725 GACGAGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1274 CCATGG C^C[GA][CT]GG
Eam1105I 127 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2742 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2770 GGTAACC G^GT[ACGT]ACC
FbaI 1079 TGATCA T^GATCA
HaeII 647 AGCGCC [GA]GCGC^[CT]
HhaI 648 GCGC GCG^C
HinP1I 648 GCGC G^CGC
MslI 1192 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 158 CGCG CG^CG
NcoI 1274 CCATGG C^CATGG
NspI 3106 ACATGC [GA]CATG^[CT]
PshAI 687 GACGATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1184 TCATGA T^CATGA
SalI 3102 GTCGAC G^TCGAC
SexAI 1093 ACCTGGT A^CC[AT]GGT
XbaI 363 TCTAGA T^CTAGA
XcmI 991 CCATGTACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1054 GAATTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 34 GGTACC G^GTACC
1004 GGTACC
AflIII 1713 ACACGT A^C[GA][CT]GT
2514 ACACGT
Asp718I 34 GGTACC G^GTACC
1004 GGTACC
AspHI 1012 GAGCTC G[AT]GC[AT]^C
2617 GAGCTC
BalI 1426 TGGCCA TGG^CCA
1457 TGGCCA
BanI 34 GGTACC G^G[CT][GA]CC
1004 GGTACC
BciVI 3060 GGATAC GTATCC(6/5)
161 GTATCC
BglII 360 AGATCT A^GATCT
1107 AGATCT
BsaAI 601 CACGTA [CT]AC^GT[GA]
2515 CACGTA
BsaBI 73 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1080 GATCACGATC
BsgI 748 CTGCAC GTGCAG(16/14)
325 GTGCAG
BsiHKAI 1012 GAGCTC G[AT]GC[AT]^C
2617 GAGCTC
BspMI 19 ACCTGC ACCTGC(4/8)
2774 ACCTGC
DraIII 287 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2933 CACATCGTG
EaeI 1426 TGGCCA [CT]^GGCC[GA]
1457 TGGCCA
EcoRI 91 GAATTC G^AATTC
1663 GAATTC
FauI 156 CCCGC CCCGC(4/6)
2644 CCCGC
HincII 1981 GTTGAC GT[CT]^[GA]AC
3102 GTCGAC
HindII 1981 GTTGAC GT[CT]^[GA]AC
3102 GTCGAC
KpnI 34 GGTACC GGTAC^C
1004 GGTACC
MamI 73 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1080 GATCACGATC
MluNI 1426 TGGCCA TGG^CCA
1457 TGGCCA
MscI 1426 TGGCCA TGG^CCA
1457 TGGCCA
PpuMI 2315 AGGACCT [GA]G^G[AT]CC[CT]
2483 AGGACCT
SacI 1012 GAGCTC GAGCT^C
2617 GAGCTC
SspI 1558 AATATT AAT^ATT
1990 AATATT
StuI 1115 AGGCCT AGG^CCT
2750 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII