Restriction map for
pF1KB8902
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaII AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsgI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BsrI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeIII
MamI MfeI MflI MluI MseI
MunI NcoI NdeI NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccIII 12 TCCGGA T^CCGGA
AcyI 331 GGCGCC G[GA]^CG[CT]C
Aor51HI 374 AGCGCT AGC^GCT
AspHI 228 GAGCAC G[AT]GC[AT]^C
AspI 60 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 470 TGGCCA TGG^CCA
BanI 331 GGCGCC G^G[CT][GA]CC
BbeI 331 GGCGCC GGCGC^C
BlpI 225 GCTGAGC GC^T[ACGT]AGC
BpmI 684 CTGGAG CTGGAG(16/14)
Bpu1102I 225 GCTGAGC GC^T[ACGT]AGC
BsaHI 331 GGCGCC G[GA]^CG[CT]C
BsaWI 12 TCCGGA [AT]^CCGG[AT]
BseAI 12 TCCGGA T^CCGGA
BsiEI 129 CGGCCG CG[GA][CT]^CG
BsiHKAI 228 GAGCAC G[AT]GC[AT]^C
BsmBI 262 GAGACG CGTCTC(1/5)
BsmFI 107 GGGAC GGGAC(10/14)
BspEI 12 TCCGGA T^CCGGA
BsrBI 73 CCGCTC CCGCTC(-3/-3)
BsrFI 591 GCCGGC [GA]^CCGG[CT]
BssHII 113 GCGCGC G^CGCGC
BssSI 135 CACGAG CACGAG(-5/-1)
BstXI 691 CCAGCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 225 GCTGAGC GC^T[ACGT]AGC
Cfr10I 591 GCCGGC [GA]^CCGG[CT]
DpnII 408 GATC ^GATC
DraII 235 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 19 CCGCGG C^C[GA][CT]GG
EagI 129 CGGCCG C^GGCCG
EclXI 129 CGGCCG C^GGCCG
Eco47III 374 AGCGCT AGC^GCT
Eco52I 129 CGGCCG C^GGCCG
EcoO109I 235 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 340 CCAAGG C^C[AT][AT]GG
Hin1I 331 GGCGCC G[GA]^CG[CT]C
HinfI 313 GACTC G^A[ACGT]TC
HphI 405 GGTGA GGTGA(8/7)
KasI 331 GGCGCC G^GCGCC
KspI 19 CCGCGG CCGC^GG
MaeII 79 ACGT A^CGT
MboI 408 GATC ^GATC
MboII 504 GAAGA GAAGA(8/7)
MluNI 470 TGGCCA TGG^CCA
MroI 12 TCCGGA T^CCGGA
MscI 470 TGGCCA TGG^CCA
MslI 399 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 591 GCCGGC GCC^GGC
NarI 331 GGCGCC GG^CGCC
NdeII 408 GATC ^GATC
NgoMIV 591 GCCGGC G^CCGGC
NotI 128 GCGGCCGC GC^GGCCGC
PflFI 60 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 313 GACTC GAGTC(4/5)
SacII 19 CCGCGG CCGC^GG
Sau3AI 408 GATC ^GATC
SfiI 462 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 331 GGCGCC GGC^GCC
SmlI 481 CTCAAG C^T[CT][GA]AG
StuI 608 AGGCCT AGG^CCT
StyI 340 CCAAGG C^C[AT][AT]GG
TaqI 410 TCGA T^CGA
Tth111I 60 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 410 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 441 GTAC GT^AC
669 GTAC
ApaI 235 GGGCCC GGGCC^C
461 GGGCCC
BglI 282 GCCAGAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
463 GCCCACCTGGC
BstF5I 90 CATCC GGATG(2/0)
99 CATCC
EaeI 129 CGGCCG [CT]^GGCC[GA]
470 TGGCCA
EcoNI 87 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
597 CCTGAAAGAGG
FauI 239 CCCGC CCCGC(4/6)
105 GCGGG
FokI 90 CATCC GGATG(9/13)
99 CATCC
HaeII 331 GGCGCC [GA]GCGC^[CT]
374 AGCGCT
PvuII 223 CAGCTG CAG^CTG
681 CAGCTG
RsaI 441 GTAC GT^AC
669 GTAC
SmaI 25 CCCGGG CCC^GGG
617 CCCGGG
XmaCI 25 CCCGGG C^CCGGG
617 CCCGGG
XmaI 25 CCCGGG C^CCGGG
617 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI AvaI BanII
BbvI BcnI BmyI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsoBI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
Sau96I ScrFI TseI