Restriction map for
pF1KB8950
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbvCI BclI BfrI
BglI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 482 GGTACC G^GTACC
AfaI 483 GTAC GT^AC
AlwI 650 GGATC GGATC(4/5)
AlwNI 598 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 172 GGGCCC GGGCC^C
Asp718I 482 GGTACC G^GTACC
AvrII 690 CCTAGG C^CTAGG
BalI 512 TGGCCA TGG^CCA
BbrPI 281 CACGTG CAC^GTG
BbsI 369 GAAGAC GAAGAC(2/6)
BcgI 177 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 959 GTATCC GTATCC(6/5)
BglII 548 AGATCT A^GATCT
BlnI 690 CCTAGG C^CTAGG
BpuAI 369 GAAGAC GAAGAC(2/6)
BsaAI 281 CACGTG [CT]AC^GT[GA]
BseRI 129 CTCCTC GAGGAG(10/8)
BsgI 807 CTGCAC GTGCAG(16/14)
BsmI 900 GAATGC GAATGC(1/-1)
BsrI 661 CCAGT ACTGG(1/-1)
BstAPI 680 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 48 CATCC GGATG(2/0)
BstYI 548 AGATCT [GA]^GATC[CT]
DdeI 36 CTCAG C^T[ACGT]AG
DraII 311 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 430 CTCTTC CTCTTC(1/4)
EcoO109I 311 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 48 CATCC GGATG(9/13)
HphI 232 TCACC GGTGA(8/7)
KpnI 482 GGTACC GGTAC^C
Ksp632I 430 CTCTTC CTCTTC(1/4)
MaeIII 773 GTTAC ^GT[ACGT]AC
MflI 548 AGATCT [GA]^GATC[CT]
MluNI 512 TGGCCA TGG^CCA
MscI 512 TGGCCA TGG^CCA
NaeI 846 GCCGGC GCC^GGC
NgoMIV 846 GCCGGC G^CCGGC
NspI 137 GCATGC [GA]CATG^[CT]
PflMI 597 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 281 CACGTG CAC^GTG
PmlI 281 CACGTG CAC^GTG
PpuMI 311 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 483 GTAC GT^AC
SacI 94 GAGCTC GAGCT^C
SapI 429 GCTCTTC GCTCTTC(1/4)
SphI 137 GCATGC GCATG^C
StuI 161 AGGCCT AGG^CCT
TfiI 921 GATTC G^A[AT]TC
Van91I 597 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 548 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 94 GAGCTC G[AT]GC[AT]^C
427 GTGCTC
AvaII 312 GGACC G^G[AT]CC
594 GGACC
BbeI 123 GGCGCC GGCGC^C
702 GGCGCC
BfaI 317 CTAG C^TAG
691 CTAG
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
427 GTGCTC
BsmFI 695 GGGAC GGGAC(10/14)
893 GGGAC
BspMI 444 GCAGGT ACCTGC(4/8)
540 GCAGGT
BsrBI 391 GAGCGG CCGCTC(-3/-3)
460 GAGCGG
DpnII 549 GATC ^GATC
651 GATC
EcoT14I 308 CCAAGG C^C[AT][AT]GG
690 CCTAGG
HgaI 247 GCGTC GACGC(5/10)
887 GCGTC
KasI 123 GGCGCC G^GCGCC
702 GGCGCC
MaeI 317 CTAG C^TAG
691 CTAG
MaeII 282 ACGT A^CGT
532 ACGT
MboI 549 GATC ^GATC
651 GATC
MboII 369 GAAGA GAAGA(8/7)
431 TCTTC
NarI 123 GGCGCC GG^CGCC
702 GGCGCC
NdeII 549 GATC ^GATC
651 GATC
PleI 600 GACTC GAGTC(4/5)
697 GACTC
PstI 346 CTGCAG CTGCA^G
789 CTGCAG
PvuII 508 CAGCTG CAG^CTG
804 CAGCTG
Sau3AI 549 GATC ^GATC
651 GATC
SfoI 123 GGCGCC GGC^GCC
702 GGCGCC
SmaI 398 CCCGGG CCC^GGG
916 CCCGGG
StyI 308 CCAAGG C^C[AT][AT]GG
690 CCTAGG
XmaCI 398 CCCGGG C^CCGGG
916 CCCGGG
XmaI 398 CCCGGG C^CCGGG
916 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BsrFI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I EaeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV Sau96I ScrFI
SfcI TseI TspRI