Restriction map for
pF1KB6774
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# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 24 GATCC GGATC(4/5)
ApaI 363 GGGCCC GGGCC^C
AspHI 64 GAGCAC G[AT]GC[AT]^C
AvaI 16 CCCGAG C^[CT]CG[GA]G
AvaII 186 GGACC G^G[AT]CC
BanI 70 GGCACC G^G[CT][GA]CC
BbsI 238 GAAGAC GAAGAC(2/6)
BglI 382 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 306 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 306 GCTCAGC GC^T[ACGT]AGC
BpuAI 238 GAAGAC GAAGAC(2/6)
BsgI 421 GTGCAG GTGCAG(16/14)
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
BsiYI 288 CCGGGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 288 CCGGGCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 16 CCCGAG C^[CT]CG[GA]G
BspMI 110 ACCTGC ACCTGC(4/8)
BssSI 208 CTCGTG CACGAG(-5/-1)
BstF5I 444 GGATG GGATG(2/0)
CelII 306 GCTCAGC GC^T[ACGT]AGC
CfoI 6 GCGC GCG^C
DpnII 24 GATC ^GATC
DraII 363 GGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 121 CTCTTC CTCTTC(1/4)
EcoO109I 363 GGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 444 GGATG GGATG(9/13)
HaeII 5 AGCGCC [GA]GCGC^[CT]
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
Ksp632I 121 CTCTTC CTCTTC(1/4)
MaeIII 226 GTGAC ^GT[ACGT]AC
MboI 24 GATC ^GATC
MspA1I 58 CCGCTG C[AC]G^C[GT]G
NdeII 24 GATC ^GATC
NspI 344 ACATGC [GA]CATG^[CT]
PstI 221 CTGCAG CTGCA^G
Sau3AI 24 GATC ^GATC
SfcI 221 CTGCAG C^T[GA][CT]AG
Tsp45I 226 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 129 GTAC GT^AC
261 GTAC
BanII 13 GAGCCC G[GA]GC[CT]^C
363 GGGCCC
BcnI 9 CCCGG CC^[GC]GG
288 CCGGG
BsaJI 98 CCCTGG C^C[ACGT][ACGT]GG
273 CCTGGG
BseRI 168 CTCCTC GAGGAG(10/8)
256 GAGGAG
BsmAI 164 GTCTC GTCTC(1/5)
333 GTCTC
DdeI 176 CTGAG C^T[ACGT]AG
307 CTCAG
FauI 57 CCCGC CCCGC(4/6)
39 GCGGG
HinfI 331 GAGTC G^A[ACGT]TC
432 GAGTC
HphI 201 GGTGA GGTGA(8/7)
328 GGTGA
MboII 238 GAAGA GAAGA(8/7)
122 TCTTC
NciI 9 CCCGG CC^[GC]GG
288 CCGGG
NlaIII 345 CATG CATG^
429 CATG
PleI 331 GAGTC GAGTC(4/5)
432 GAGTC
RsaI 129 GTAC GT^AC
261 GTAC
SfaNI 204 GATGC GCATC(5/9)
217 GATGC
TaqI 319 TCGA T^CGA
435 TCGA
TthHB8I 319 TCGA T^CGA
435 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BmyI BpmI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MnlI MspI MvaI
MwoI NlaIV Sau96I ScrFI TseI
TspRI