Restriction map for
pF1KB6482
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII BalI BamHI BbvCI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaBI BsaI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I CelII ClaI CpoI
DpnII DraI DraIII DrdI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI HphI KpnI KspI
MamI MboI MfeI MflI MluI
MluNI MscI MseI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 75 TCCGGA T^CCGGA
Alw44I 881 GTGCAC G^TGCAC
AocI 780 CCTGAGG CC^T[ACGT]AGG
ApaLI 881 GTGCAC G^TGCAC
AspHI 881 GTGCAC G[AT]GC[AT]^C
AvrII 276 CCTAGG C^CTAGG
BbsI 148 GTCTTC GAAGAC(2/6)
BcgI 824 GCACCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 276 CCTAGG C^CTAGG
BpuAI 148 GTCTTC GAAGAC(2/6)
BseAI 75 TCCGGA T^CCGGA
BsiHKAI 881 GTGCAC G[AT]GC[AT]^C
BsmBI 959 GAGACG CGTCTC(1/5)
BsmFI 173 GGGAC GGGAC(10/14)
BspEI 75 TCCGGA T^CCGGA
BspMI 263 ACCTGC ACCTGC(4/8)
BsrFI 244 GCCGGC [GA]^CCGG[CT]
BsrI 584 ACTGG ACTGG(1/-1)
Bsu36I 780 CCTGAGG CC^T[ACGT]AGG
Cfr10I 244 GCCGGC [GA]^CCGG[CT]
DsaI 323 CCACGG C^C[GA][CT]GG
Eco81I 780 CCTGAGG CC^T[ACGT]AGG
EcoNI 483 CCTCGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 276 CCTAGG C^C[AT][AT]GG
MaeIII 160 GTCAC ^GT[ACGT]AC
MroI 75 TCCGGA T^CCGGA
NaeI 244 GCCGGC GCC^GGC
NgoMIV 244 GCCGGC G^CCGGC
NspI 809 GCATGT [GA]CATG^[CT]
PpuMI 96 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 595 CAGCTG CAG^CTG
SfaNI 682 GATGC GCATC(5/9)
SmaI 123 CCCGGG CCC^GGG
StyI 276 CCTAGG C^C[AT][AT]GG
Tsp45I 160 GTCAC ^GT[GC]AC
XmaCI 123 CCCGGG C^CCGGG
XmaI 123 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 349 GGCGCC G[GA]^CG[CT]C
455 GGCGCC
ApaI 120 GGGCCC GGGCC^C
492 GGGCCC
AvaI 123 CCCGGG C^[CT]CG[GA]G
901 CCCGAG
BanI 349 GGCGCC G^G[CT][GA]CC
455 GGCGCC
BbeI 349 GGCGCC GGCGC^C
455 GGCGCC
BbrPI 634 CACGTG CAC^GTG
714 CACGTG
BfaI 232 CTAG C^TAG
277 CTAG
BsaAI 634 CACGTG [CT]AC^GT[GA]
714 CACGTG
BsaHI 349 GGCGCC G[GA]^CG[CT]C
455 GGCGCC
BsaWI 75 TCCGGA [AT]^CCGG[AT]
705 ACCGGA
BsiEI 242 CGGCCG CG[GA][CT]^CG
720 CGGCCG
BsmAI 316 GAGAC GTCTC(1/5)
959 GAGAC
BsoBI 123 CCCGGG C^[CT]CG[GA]G
901 CCCGAG
EagI 242 CGGCCG C^GGCCG
720 CGGCCG
EarI 1043 CTCTTC CTCTTC(1/4)
565 GAAGAG
EclXI 242 CGGCCG C^GGCCG
720 CGGCCG
Eco52I 242 CGGCCG C^GGCCG
720 CGGCCG
HgaI 34 GACGC GACGC(5/10)
79 GACGC
Hin1I 349 GGCGCC G[GA]^CG[CT]C
455 GGCGCC
HinfI 198 GATTC G^A[ACGT]TC
544 GATTC
KasI 349 GGCGCC G^GCGCC
455 GGCGCC
Ksp632I 1043 CTCTTC CTCTTC(1/4)
565 GAAGAG
MaeI 232 CTAG C^TAG
277 CTAG
NarI 349 GGCGCC GG^CGCC
455 GGCGCC
PmaCI 634 CACGTG CAC^GTG
714 CACGTG
PmlI 634 CACGTG CAC^GTG
714 CACGTG
SfoI 349 GGCGCC GGC^GCC
455 GGCGCC
TfiI 198 GATTC G^A[AT]TC
544 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfcI
TseI TspRI