Restriction map for
pF1KB8977
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
MamI MfeI MluI MroI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tsp509I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 767 GGTACC G^GTACC
AlwI 280 GATCC GGATC(4/5)
AocI 623 CCTCAGG CC^T[ACGT]AGG
Aor51HI 792 AGCGCT AGC^GCT
ApaI 929 GGGCCC GGGCC^C
Asp718I 767 GGTACC G^GTACC
AspHI 250 GAGCTC G[AT]GC[AT]^C
AvaI 1091 CCCGGG C^[CT]CG[GA]G
BanI 767 GGTACC G^G[CT][GA]CC
BbsI 418 GAAGAC GAAGAC(2/6)
BlpI 640 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 640 GCTAAGC GC^T[ACGT]AGC
BpuAI 418 GAAGAC GAAGAC(2/6)
BsaI 598 GAGACC GGTCTC(1/5)
BsaWI 947 ACCGGA [AT]^CCGG[AT]
BseRI 621 CTCCTC GAGGAG(10/8)
BsiHKAI 250 GAGCTC G[AT]GC[AT]^C
BsmI 120 GAATGC GAATGC(1/-1)
BsoBI 1091 CCCGGG C^[CT]CG[GA]G
BsrFI 735 GCCGGT [GA]^CCGG[CT]
BstXI 557 CCAACTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 279 AGATCC [GA]^GATC[CT]
Bsu36I 623 CCTCAGG CC^T[ACGT]AGG
CelII 640 GCTAAGC GC^T[ACGT]AGC
Cfr10I 735 GCCGGT [GA]^CCGG[CT]
DrdI 718 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 933 CCGCGG C^C[GA][CT]GG
EarI 1042 GAAGAG CTCTTC(1/4)
Eco47III 792 AGCGCT AGC^GCT
Eco81I 623 CCTCAGG CC^T[ACGT]AGG
HaeII 792 AGCGCT [GA]GCGC^[CT]
HincII 727 GTCAAC GT[CT]^[GA]AC
HindII 727 GTCAAC GT[CT]^[GA]AC
KpnI 767 GGTACC GGTAC^C
Ksp632I 1042 GAAGAG CTCTTC(1/4)
KspI 933 CCGCGG CCGC^GG
MaeII 681 ACGT A^CGT
MaeIII 992 GTGAC ^GT[ACGT]AC
MflI 279 AGATCC [GA]^GATC[CT]
MseI 749 TTAA T^TAA
NheI 789 GCTAGC G^CTAGC
PstI 109 CTGCAG CTGCA^G
SacI 250 GAGCTC GAGCT^C
SacII 933 CCGCGG CCGC^GG
SmaI 1091 CCCGGG CCC^GGG
SmlI 472 CTTGAG C^T[CT][GA]AG
StuI 339 AGGCCT AGG^CCT
Tru9I 749 TTAA T^TAA
Tsp45I 992 GTGAC ^GT[GC]AC
XhoII 279 AGATCC [GA]^GATC[CT]
XmaCI 1091 CCCGGG C^CCGGG
XmaI 1091 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 934 CGCG CG^CG
1050 CGCG
BalI 305 TGGCCA TGG^CCA
692 TGGCCA
BglI 332 GCCTCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
393 GCCAGCAGGGC
BsgI 650 CTGCAC GTGCAG(16/14)
874 GTGCAG
BsmAI 598 GAGAC GTCTC(1/5)
316 GTCTC
BsrI 313 CCAGT ACTGG(1/-1)
529 CCAGT
BstNI 378 CCTGG CC^[AT]GG
954 CCTGG
BstUI 934 CGCG CG^CG
1050 CGCG
DpnII 90 GATC ^GATC
280 GATC
DraII 627 AGGCCCT [GA]G^G[ACGT]CC[CT]
928 AGGGCCC
EaeI 305 TGGCCA [CT]^GGCC[GA]
692 TGGCCA
EcoO109I 627 AGGCCCT [GA]G^G[ACGT]CC[CT]
928 AGGGCCC
EcoRII 378 CCTGG ^CC[AT]GG
954 CCTGG
HphI 36 GGTGA GGTGA(8/7)
101 TCACC
MboI 90 GATC ^GATC
280 GATC
MluNI 305 TGGCCA TGG^CCA
692 TGGCCA
MscI 305 TGGCCA TGG^CCA
692 TGGCCA
MvaI 378 CCTGG CC^[AT]GG
954 CCTGG
MvnI 934 CGCG CG^CG
1050 CGCG
NdeII 90 GATC ^GATC
280 GATC
PflMI 163 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
214 CCAGATTCTGG
PleI 433 GACTC GAGTC(4/5)
669 GAGTC
Sau3AI 90 GATC ^GATC
280 GATC
SfaNI 460 GATGC GCATC(5/9)
259 GCATC
SfcI 109 CTGCAG C^T[GA][CT]AG
453 CTATAG
Van91I 163 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
214 CCAGATTCTGG
XcmI 210 CCACCCAGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
869 CCATTGTGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrBI BssKI BstF5I Cac8I
CfoI Cfr13I DdeI EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MboII MnlI MspA1I MspI MwoI
NciI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI StyI TfiI TseI
TspRI