Restriction map for
pF1KB6479
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Cac8I
CelII Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MwoI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 528 GTCTAC GT^[AC][GT]AC
AccII 730 CGCG CG^CG
AcyI 510 GGCGCC G[GA]^CG[CT]C
AhdI 520 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 479 GTGCAC G^TGCAC
AocI 514 CCTCAGG CC^T[ACGT]AGG
ApaLI 479 GTGCAC G^TGCAC
AspEI 520 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 510 GGCGCC G^G[CT][GA]CC
BanII 501 GAGCTC G[GA]GC[CT]^C
BbeI 510 GGCGCC GGCGC^C
BbvI 21 GCTGC GCAGC(8/12)
BciVI 72 GTATCC GTATCC(6/5)
BglII 833 AGATCT A^GATCT
BsaAI 635 TACGTG [CT]AC^GT[GA]
BsaHI 510 GGCGCC G[GA]^CG[CT]C
BsaWI 321 ACCGGA [AT]^CCGG[AT]
BsrDI 550 GCAATG GCAATG(2/0)
BstUI 730 CGCG CG^CG
BstYI 833 AGATCT [GA]^GATC[CT]
Bsu36I 514 CCTCAGG CC^T[ACGT]AGG
CfoI 511 GCGC GCG^C
DraI 976 TTTAAA TTT^AAA
DraII 735 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 729 CCGCGG C^C[GA][CT]GG
Eam1105I 520 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 837 CTCTTC CTCTTC(1/4)
Eco81I 514 CCTCAGG CC^T[ACGT]AGG
EcoNI 149 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 735 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 555 GATATC GAT^ATC
Fnu4HI 21 GCTGC GC^[ACGT]GC
HaeII 510 GGCGCC [GA]GCGC^[CT]
HhaI 511 GCGC GCG^C
Hin1I 510 GGCGCC G[GA]^CG[CT]C
HinP1I 511 GCGC G^CGC
ItaI 21 GCTGC GC^[ACGT]GC
KasI 510 GGCGCC G^GCGCC
Ksp632I 837 CTCTTC CTCTTC(1/4)
KspI 729 CCGCGG CCGC^GG
MaeII 636 ACGT A^CGT
MflI 833 AGATCT [GA]^GATC[CT]
MspA1I 729 CCGCGG C[AC]G^C[GT]G
MvnI 730 CGCG CG^CG
NarI 510 GGCGCC GG^CGCC
PleI 526 GAGTC GAGTC(4/5)
PpuMI 735 GGGACCC [GA]G^G[AT]CC[CT]
SacI 501 GAGCTC GAGCT^C
SacII 729 CCGCGG CCGC^GG
SfcI 842 CTATAG C^T[GA][CT]AG
SfoI 510 GGCGCC GGC^GCC
TseI 21 GCTGC G^C[AT]GC
Tsp45I 412 GTGAC ^GT[GC]AC
XhoII 833 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 916 GATCC GGATC(4/5)
306 GGATC
AspHI 479 GTGCAC G[AT]GC[AT]^C
501 GAGCTC
BbsI 159 GAAGAC GAAGAC(2/6)
113 GTCTTC
BcnI 381 CCGGG CC^[GC]GG
801 CCGGG
BmyI 479 GTGCAC G[AGT]GC[ACT]^C
501 GAGCTC
BpmI 953 CTCCAG CTGGAG(16/14)
886 CTGGAG
BpuAI 159 GAAGAC GAAGAC(2/6)
113 GTCTTC
BseRI 147 CTCCTC GAGGAG(10/8)
223 GAGGAG
BsiHKAI 479 GTGCAC G[AT]GC[AT]^C
501 GAGCTC
BsmFI 735 GGGAC GGGAC(10/14)
606 GTCCC
Bsp1286I 479 GTGCAC G[AGT]GC[ACT]^C
501 GAGCTC
FauI 728 CCCGC CCCGC(4/6)
731 GCGGG
HaeIII 328 GGCC GG^CC
650 GGCC
MaeIII 412 GTGAC ^GT[ACGT]AC
713 GTTAC
MseI 962 TTAA T^TAA
977 TTAA
NciI 381 CCGGG CC^[GC]GG
801 CCGGG
TaqI 245 TCGA T^CGA
789 TCGA
Tru9I 962 TTAA T^TAA
977 TTAA
TthHB8I 245 TCGA T^CGA
789 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII EcoT14I FokI HapII
HinfI HpaII HphI MboI MboII
MnlI MspI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
StyI TfiI Tsp509I TspRI