Restriction map for
pF1KB6155
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# NO CUT ENZYMES ( 104 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MunI
NcoI NdeI NheI NotI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1672 GACGTC GACGT^C
AccIII 233 TCCGGA T^CCGGA
AfaI 468 GTAC GT^AC
ApaI 409 GGGCCC GGGCC^C
AspI 68 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 323 TGGCCA TGG^CCA
BbeI 86 GGCGCC GGCGC^C
BclI 1358 TGATCA T^GATCA
BglI 35 GCCAGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1689 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 191 GAGACC GGTCTC(1/5)
BsaWI 233 TCCGGA [AT]^CCGG[AT]
BseAI 233 TCCGGA T^CCGGA
BsiEI 1138 CGGCCG CG[GA][CT]^CG
BspEI 233 TCCGGA T^CCGGA
BspMI 242 ACCTGC ACCTGC(4/8)
BstBI 940 TTCGAA TT^CGAA
DraIII 889 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 271 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 832 CCGCGG C^C[GA][CT]GG
EagI 1138 CGGCCG C^GGCCG
EclXI 1138 CGGCCG C^GGCCG
Eco52I 1138 CGGCCG C^GGCCG
FbaI 1358 TGATCA T^GATCA
KasI 86 GGCGCC G^GCGCC
KspI 832 CCGCGG CCGC^GG
MamI 1689 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 323 TGGCCA TGG^CCA
MroI 233 TCCGGA T^CCGGA
MscI 323 TGGCCA TGG^CCA
MseI 146 TTAA T^TAA
MslI 1350 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 89 GCCGGC GCC^GGC
NarI 86 GGCGCC GG^CGCC
NgoMIV 89 GCCGGC G^CCGGC
NruI 75 TCGCGA TCG^CGA
NspV 940 TTCGAA TT^CGAA
PflFI 68 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1287 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1088 CAGCTG CAG^CTG
RsaI 468 GTAC GT^AC
SacI 1021 GAGCTC GAGCT^C
SacII 832 CCGCGG CCGC^GG
SfoI 86 GGCGCC GGC^GCC
SfuI 940 TTCGAA TT^CGAA
SgrAI 88 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1544 CCCGGG CCC^GGG
SmlI 1228 CTCAAG C^T[CT][GA]AG
Tru9I 146 TTAA T^TAA
Tth111I 68 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1544 CCCGGG C^CCGGG
XmaI 1544 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AcyI 86 GGCGCC G[GA]^CG[CT]C
1672 GACGTC
AlwI 495 GATCC GGATC(4/5)
1194 GATCC
Asp700I 210 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
618 GAAGCCCTTC
AvaI 289 CTCGGG C^[CT]CG[GA]G
1544 CCCGGG
BfaI 628 CTAG C^TAG
1744 CTAG
BsaHI 86 GGCGCC G[GA]^CG[CT]C
1672 GACGTC
BsmBI 1674 CGTCTC CGTCTC(1/5)
1780 GAGACG
BsoBI 289 CTCGGG C^[CT]CG[GA]G
1544 CCCGGG
BsrBI 930 CCGCTC CCGCTC(-3/-3)
976 GAGCGG
BstYI 494 AGATCC [GA]^GATC[CT]
1193 AGATCC
EaeI 323 TGGCCA [CT]^GGCC[GA]
1138 CGGCCG
EarI 25 CTCTTC CTCTTC(1/4)
817 CTCTTC
EcoNI 540 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1279 CCTGGGCAAGG
EcoT14I 186 CCAAGG C^C[AT][AT]GG
1008 CCAAGG
HgaI 415 GACGC GACGC(5/10)
1729 GACGC
Hin1I 86 GGCGCC G[GA]^CG[CT]C
1672 GACGTC
Ksp632I 25 CTCTTC CTCTTC(1/4)
817 CTCTTC
MaeI 628 CTAG C^TAG
1744 CTAG
MaeIII 655 GTCAC ^GT[ACGT]AC
1073 GTGAC
MflI 494 AGATCC [GA]^GATC[CT]
1193 AGATCC
SapI 24 GCTCTTC GCTCTTC(1/4)
816 GCTCTTC
SfaNI 716 GCATC GCATC(5/9)
1267 GCATC
StyI 186 CCAAGG C^C[AT][AT]GG
1008 CCAAGG
TaqI 616 TCGA T^CGA
941 TCGA
TfiI 138 GAATC G^A[AT]TC
747 GATTC
Tsp45I 655 GTCAC ^GT[GC]AC
1073 GTGAC
TthHB8I 616 TCGA T^CGA
941 TCGA
XcmI 1422 CCAGGGCCAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1701 CCAGGAGGAGGGTGG
XhoII 494 AGATCC [GA]^GATC[CT]
1193 AGATCC
XmnI 210 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
618 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
Sau3AI Sau96I ScrFI SfcI TseI
TspRI Van91I