Restriction map for
pF1KE0030
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# NO CUT ENZYMES ( 85 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstZ17I ClaI CpoI DraI DraIII
DrdI EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
KpnI MaeI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SapI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI StuI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 331 GACGTC GACGT^C
AflIII 935 ACGTGT A^C[GA][CT]GT
AhdI 1168 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1036 GTGCAC G^TGCAC
AlwNI 1627 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1015 CCTGAGG CC^T[ACGT]AGG
ApaLI 1036 GTGCAC G^TGCAC
AspEI 1168 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 494 TGCGCA TGC^GCA
BalI 371 TGGCCA TGG^CCA
BanII 465 GGGCTC G[GA]GC[CT]^C
BbrPI 934 CACGTG CAC^GTG
BbvCI 1392 CCTCAGC CCTCAGC(-5/-2)
BglI 1582 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 204 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 204 GCTGAGC GC^T[ACGT]AGC
BsaBI 1299 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 379 GAGACC GGTCTC(1/5)
BsiEI 1529 CGGCCG CG[GA][CT]^CG
BsrBI 693 GAGCGG CCGCTC(-3/-3)
BssHII 181 GCGCGC G^CGCGC
BstEII 525 GGTCACC G^GT[ACGT]ACC
BstPI 525 GGTCACC G^GT[ACGT]ACC
BstXI 305 CCAGGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1304 AGATCC [GA]^GATC[CT]
Bsu36I 1015 CCTGAGG CC^T[ACGT]AGG
CelII 204 GCTGAGC GC^T[ACGT]AGC
EagI 1529 CGGCCG C^GGCCG
Eam1105I 1168 GACGTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 727 CTCTTC CTCTTC(1/4)
EclXI 1529 CGGCCG C^GGCCG
Eco52I 1529 CGGCCG C^GGCCG
Eco81I 1015 CCTGAGG CC^T[ACGT]AGG
EcoO65I 525 GGTCACC G^GT[ACGT]ACC
FspI 494 TGCGCA TGC^GCA
Ksp632I 727 CTCTTC CTCTTC(1/4)
KspI 1354 CCGCGG CCGC^GG
MamI 1299 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1304 AGATCC [GA]^GATC[CT]
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MseI 806 TTAA T^TAA
NaeI 1527 GCCGGC GCC^GGC
NgoMIV 1527 GCCGGC G^CCGGC
PaeR7I 1235 CTCGAG C^TCGAG
PflFI 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 821 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 934 CACGTG CAC^GTG
PmlI 934 CACGTG CAC^GTG
SacII 1354 CCGCGG CCGC^GG
SbfI 132 CCTGCAGG CCTGCA^GG
ScaI 486 AGTACT AGT^ACT
SmaI 1178 CCCGGG CCC^GGG
SmlI 1235 CTCGAG C^T[CT][GA]AG
SphI 116 GCATGC GCATG^C
Sse8387I 132 CCTGCAGG CCTGCA^GG
TfiI 1186 GATTC G^A[AT]TC
Tru9I 806 TTAA T^TAA
Tsp509I 344 AATT ^AATT
Tth111I 331 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 821 CCAAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1235 CTCGAG C^TCGAG
XhoII 1304 AGATCC [GA]^GATC[CT]
XmaCI 1178 CCCGGG C^CCGGG
XmaI 1178 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Aor51HI 824 AGCGCT AGC^GCT
1085 AGCGCT
BbeI 66 GGCGCC GGCGC^C
283 GGCGCC
BsaAI 934 CACGTG [CT]AC^GT[GA]
1585 TACGTG
BseRI 1609 CTCCTC GAGGAG(10/8)
794 GAGGAG
BsgI 925 CTGCAC GTGCAG(16/14)
931 CTGCAC
BsmBI 699 CGTCTC CGTCTC(1/5)
991 GAGACG
BsmI 159 GAATGC GAATGC(1/-1)
1509 GAATGC
BsrDI 1218 CATTGC GCAATG(2/0)
1560 CATTGC
BsrFI 1281 GCCGGT [GA]^CCGG[CT]
1527 GCCGGC
BstF5I 297 CATCC GGATG(2/0)
1095 CATCC
Cfr10I 1281 GCCGGT [GA]^CCGG[CT]
1527 GCCGGC
Eco47III 824 AGCGCT AGC^GCT
1085 AGCGCT
EcoNI 864 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1053 CCTGGCGGAGG
FokI 297 CATCC GGATG(9/13)
1095 CATCC
KasI 66 GGCGCC G^GCGCC
283 GGCGCC
MslI 1164 CATGGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1278 CATGCCGGTG
NarI 66 GGCGCC GG^CGCC
283 GGCGCC
NspI 116 GCATGC [GA]CATG^[CT]
1277 ACATGC
PpuMI 608 AGGACCT [GA]G^G[AT]CC[CT]
1466 GGGACCC
PshAI 381 GACCGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1233 GACTCGAGTC
PstI 133 CTGCAG CTGCA^G
964 CTGCAG
SfcI 133 CTGCAG C^T[GA][CT]AG
964 CTGCAG
SfoI 66 GGCGCC GGC^GCC
283 GGCGCC
TaqI 356 TCGA T^CGA
1236 TCGA
TspRI 164 CAGTG CA[GC]TG[ACGT][ACGT]^
504 CACTG
TthHB8I 356 TCGA T^CGA
1236 TCGA
XcmI 620 CCATCGTCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
821 CCAAGCGCTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TseI Tsp45I