Restriction map for
pF1KB6577
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstUI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1012 GTCTAC GT^[AC][GT]AC
AciI 329 CCGC CCGC(-3/-1)
AlwNI 260 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 221 CCTTAGG CC^T[ACGT]AGG
AspHI 182 GTGCTC G[AT]GC[AT]^C
BanI 1074 GGCACC G^G[CT][GA]CC
BciVI 216 GGATAC GTATCC(6/5)
BmyI 182 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 182 GTGCTC G[AT]GC[AT]^C
BsmBI 307 CGTCTC CGTCTC(1/5)
BsmFI 388 GGGAC GGGAC(10/14)
Bsp1286I 182 GTGCTC G[AGT]GC[ACT]^C
BstBI 122 TTCGAA TT^CGAA
BstEII 505 GGTGACC G^GT[ACGT]ACC
BstPI 505 GGTGACC G^GT[ACGT]ACC
BstXI 679 CCAGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 221 CCTTAGG CC^T[ACGT]AGG
Cfr13I 42 GGCCC G^G[ACGT]CC
DraI 988 TTTAAA TTT^AAA
DraIII 791 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1043 CCATGG C^C[GA][CT]GG
Eco81I 221 CCTTAGG CC^T[ACGT]AGG
EcoO65I 505 GGTGACC G^GT[ACGT]ACC
FauI 328 CCCGC CCCGC(4/6)
HincII 292 GTTGAC GT[CT]^[GA]AC
HindII 292 GTTGAC GT[CT]^[GA]AC
MaeII 306 ACGT A^CGT
NcoI 1043 CCATGG C^CATGG
NspV 122 TTCGAA TT^CGAA
Sau96I 42 GGCCC G^G[ACGT]CC
SfuI 122 TTCGAA TT^CGAA
StuI 808 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 583 GTAC GT^AC
724 GTAC
AluI 439 AGCT AG^CT
604 AGCT
BbvI 538 GCAGC GCAGC(8/12)
1026 GCAGC
BfaI 251 CTAG C^TAG
721 CTAG
BsmAI 1006 GAGAC GTCTC(1/5)
308 GTCTC
BsrI 562 ACTGG ACTGG(1/-1)
820 ACTGG
BstAPI 268 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
567 GCAAGTGTTGC
Cac8I 327 GCCCGC GC[ACGT]^[ACGT]GC
832 GCTGGC
DpnII 399 GATC ^GATC
702 GATC
EcoT14I 841 CCTTGG C^C[AT][AT]GG
1043 CCATGG
Fnu4HI 538 GCAGC GC^[ACGT]GC
1026 GCAGC
HaeIII 42 GGCC GG^CC
809 GGCC
ItaI 538 GCAGC GC^[ACGT]GC
1026 GCAGC
MaeI 251 CTAG C^TAG
721 CTAG
MboI 399 GATC ^GATC
702 GATC
NdeII 399 GATC ^GATC
702 GATC
NspI 323 ACATGC [GA]CATG^[CT]
576 GCATGT
RsaI 583 GTAC GT^AC
724 GTAC
Sau3AI 399 GATC ^GATC
702 GATC
StyI 841 CCTTGG C^C[AT][AT]GG
1043 CCATGG
TaqI 123 TCGA T^CGA
632 TCGA
TseI 538 GCAGC G^C[AT]GC
1026 GCAGC
TthHB8I 123 TCGA T^CGA
632 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI ApoI BsaJI BssKI BstF5I
BstNI DdeI EcoRII FokI HinfI
HphI MaeIII MboII MnlI MseI
MvaI MwoI NlaIII NlaIV ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI