Restriction map for
pF1KE0169
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
HphI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 321 GTAC GT^AC
AlwI 78 GATCC GGATC(4/5)
AlwNI 295 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 159 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 558 CCCGAG C^[CT]CG[GA]G
BbsI 202 GTCTTC GAAGAC(2/6)
BbvI 21 GCTGC GCAGC(8/12)
BcnI 27 CCCGG CC^[GC]GG
BfaI 364 CTAG C^TAG
BglII 452 AGATCT A^GATCT
BpmI 289 CTGGAG CTGGAG(16/14)
BpuAI 202 GTCTTC GAAGAC(2/6)
BseRI 512 GAGGAG GAGGAG(10/8)
BsgI 373 GTGCAG GTGCAG(16/14)
BsmBI 479 GAGACG CGTCTC(1/5)
BsmFI 232 GGGAC GGGAC(10/14)
BsoBI 558 CCCGAG C^[CT]CG[GA]G
BsrFI 568 GCCGGC [GA]^CCGG[CT]
BstAPI 39 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 568 GCCGGC [GA]^CCGG[CT]
Cfr13I 308 GGGCC G^G[ACGT]CC
DsaI 494 CCATGG C^C[GA][CT]GG
EaeI 570 CGGCCA [CT]^GGCC[GA]
EcoRI 250 GAATTC G^AATTC
EcoT14I 494 CCATGG C^C[AT][AT]GG
Fnu4HI 21 GCTGC GC^[ACGT]GC
HincII 426 GTCAAC GT[CT]^[GA]AC
HindII 426 GTCAAC GT[CT]^[GA]AC
HinfI 424 GAGTC G^A[ACGT]TC
ItaI 21 GCTGC GC^[ACGT]GC
MaeI 364 CTAG C^TAG
MaeIII 271 GTAAC ^GT[ACGT]AC
MseI 143 TTAA T^TAA
NaeI 568 GCCGGC GCC^GGC
NciI 27 CCCGG CC^[GC]GG
NcoI 494 CCATGG C^CATGG
NgoMIV 568 GCCGGC G^CCGGC
NlaIV 514 GGAGCC GG[ACGT]^[ACGT]CC
PleI 424 GAGTC GAGTC(4/5)
PshAI 122 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 547 CTGCAG CTGCA^G
RsaI 321 GTAC GT^AC
Sau96I 308 GGGCC G^G[ACGT]CC
SfcI 547 CTGCAG C^T[GA][CT]AG
StyI 494 CCATGG C^C[AT][AT]GG
Tru9I 143 TTAA T^TAA
TseI 21 GCTGC G^C[AT]GC
XmnI 159 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 207 CCGC CCGC(-3/-1)
459 CCGC
AcsI 187 AAATTT [GA]^AATT[CT]
250 GAATTC
ApoI 187 AAATTT [GA]^AATT[CT]
250 GAATTC
BsaJI 494 CCATGG C^C[ACGT][ACGT]GG
510 CCGAGG
BsiYI 168 CCCTTATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCGCCAGATGG
BslI 168 CCCTTATGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
459 CCGCCAGATGG
BsmAI 107 GAGAC GTCTC(1/5)
479 GAGAC
BsrI 116 CCAGT ACTGG(1/-1)
573 CCAGT
BstF5I 507 CATCC GGATG(2/0)
177 GGATG
BstNI 81 CCAGG CC^[AT]GG
327 CCTGG
BstYI 77 AGATCC [GA]^GATC[CT]
452 AGATCT
CfoI 24 GCGC GCG^C
540 GCGC
EarI 136 GAAGAG CTCTTC(1/4)
499 GAAGAG
EcoRII 81 CCAGG ^CC[AT]GG
327 CCTGG
FokI 507 CATCC GGATG(9/13)
177 GGATG
HaeIII 309 GGCC GG^CC
571 GGCC
HapII 28 CCGG C^CGG
569 CCGG
HhaI 24 GCGC GCG^C
540 GCGC
HinP1I 24 GCGC G^CGC
540 GCGC
HpaII 28 CCGG C^CGG
569 CCGG
Ksp632I 136 GAAGAG CTCTTC(1/4)
499 GAAGAG
MflI 77 AGATCC [GA]^GATC[CT]
452 AGATCT
MspI 28 CCGG C^CGG
569 CCGG
MvaI 81 CCAGG CC^[AT]GG
327 CCTGG
NspI 198 GCATGT [GA]CATG^[CT]
317 GCATGT
SmlI 102 CTTGAG C^T[CT][GA]AG
264 CTTGAG
TaqI 215 TCGA T^CGA
277 TCGA
Tsp509I 188 AATT ^AATT
251 AATT
TthHB8I 215 TCGA T^CGA
277 TCGA
XhoII 77 AGATCC [GA]^GATC[CT]
452 AGATCT
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BssKI Cac8I DpnII MboI
MboII MnlI MwoI NdeII NlaIII
Sau3AI ScrFI SfaNI TspRI