Restriction map for
pF1KE0427
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI BspDI BspEI
BspHI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI HinP1I HindIII
HpaII HphI KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 39 CCGC CCGC(-3/-1)
AcsI 244 GAATTT [GA]^AATT[CT]
AcyI 251 GACGCC G[GA]^CG[CT]C
AflIII 362 ACATGT A^C[GA][CT]GT
ApoI 244 GAATTT [GA]^AATT[CT]
AseI 156 ATTAAT AT^TAAT
AsnI 156 ATTAAT AT^TAAT
BalI 115 TGGCCA TGG^CCA
BamHI 60 GGATCC G^GATCC
BanI 141 GGCACC G^G[CT][GA]CC
BbsI 523 GAAGAC GAAGAC(2/6)
BbvI 265 GCTGC GCAGC(8/12)
BciVI 474 GGATAC GTATCC(6/5)
BmyI 214 GGGCAC G[AGT]GC[ACT]^C
BpuAI 523 GAAGAC GAAGAC(2/6)
BsaHI 251 GACGCC G[GA]^CG[CT]C
BseRI 289 GAGGAG GAGGAG(10/8)
Bsp1286I 214 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 365 TGTACA T^GTACA
BspLU11I 362 ACATGT A^CATGT
BspMI 22 ACCTGC ACCTGC(4/8)
BsrGI 365 TGTACA T^GTACA
BsrI 113 ACTGG ACTGG(1/-1)
BstF5I 508 CATCC GGATG(2/0)
Cac8I 418 GCCAGC GC[ACGT]^[ACGT]GC
Cfr13I 14 GGGCC G^G[ACGT]CC
DraIII 143 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 10 CCACGG C^C[GA][CT]GG
EcoT14I 255 CCTTGG C^C[AT][AT]GG
Fnu4HI 265 GCTGC GC^[ACGT]GC
FokI 508 CATCC GGATG(9/13)
Hin1I 251 GACGCC G[GA]^CG[CT]C
HincII 358 GTTAAC GT[CT]^[GA]AC
HindII 358 GTTAAC GT[CT]^[GA]AC
HinfI 84 GATTC G^A[ACGT]TC
HpaI 358 GTTAAC GTT^AAC
ItaI 265 GCTGC GC^[ACGT]GC
MaeII 218 ACGT A^CGT
MluNI 115 TGGCCA TGG^CCA
MscI 115 TGGCCA TGG^CCA
NlaIII 363 CATG CATG^
NspI 362 ACATGT [GA]CATG^[CT]
PshBI 156 ATTAAT AT^TAAT
Sau96I 14 GGGCC G^G[ACGT]CC
ScaI 407 AGTACT AGT^ACT
SexAI 144 ACCTGGT A^CC[AT]GGT
SfcI 381 CTACAG C^T[GA][CT]AG
SspBI 365 TGTACA T^GTACA
StuI 517 AGGCCT AGG^CCT
StyI 255 CCTTGG C^C[AT][AT]GG
TaqI 249 TCGA T^CGA
TfiI 84 GATTC G^A[AT]TC
TseI 265 GCTGC G^C[AT]GC
Tsp45I 220 GTCAC ^GT[GC]AC
TthHB8I 249 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 366 GTAC GT^AC
408 GTAC
AluI 195 AGCT AG^CT
225 AGCT
BfaI 197 CTAG C^TAG
286 CTAG
BssKI 145 CCTGG ^CC[ACGT]GG
470 CCAGG
BstNI 145 CCTGG CC^[AT]GG
470 CCAGG
BstYI 60 GGATCC [GA]^GATC[CT]
324 GGATCT
DdeI 189 CTCAG C^T[ACGT]AG
479 CTGAG
DpnII 61 GATC ^GATC
325 GATC
EaeI 115 TGGCCA [CT]^GGCC[GA]
416 CGGCCA
EcoRII 145 CCTGG ^CC[AT]GG
470 CCAGG
HgaI 251 GACGC GACGC(5/10)
442 GACGC
MaeI 197 CTAG C^TAG
286 CTAG
MaeIII 220 GTCAC ^GT[ACGT]AC
282 GTTAC
MboI 61 GATC ^GATC
325 GATC
MflI 60 GGATCC [GA]^GATC[CT]
324 GGATCT
MvaI 145 CCTGG CC^[AT]GG
470 CCAGG
NdeII 61 GATC ^GATC
325 GATC
RsaI 366 GTAC GT^AC
408 GTAC
Sau3AI 61 GATC ^GATC
325 GATC
ScrFI 145 CCTGG CC^[ACGT]GG
470 CCAGG
Tsp509I 155 AATT ^AATT
245 AATT
XhoII 60 GGATCC [GA]^GATC[CT]
324 GGATCT
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AlwI BsaJI BsiYI BslI HaeIII
MboII MnlI MseI NlaIV Tru9I
TspRI