Restriction map for
pF1KB7807
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# NO CUT ENZYMES ( 116 enzymes) #
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AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BciVI
BclI BfaI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MaeI MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 403 GGTACC G^GTACC
AccIII 758 TCCGGA T^CCGGA
AocI 649 CCTGAGG CC^T[ACGT]AGG
Asp718I 403 GGTACC G^GTACC
BbeI 290 GGCGCC GGCGC^C
BcgI 969 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 386 AGATCT A^GATCT
BlpI 942 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 942 GCTCAGC GC^T[ACGT]AGC
BsaI 175 GAGACC GGTCTC(1/5)
BsaWI 758 TCCGGA [AT]^CCGG[AT]
BseAI 758 TCCGGA T^CCGGA
BsiEI 1416 CGACCG CG[GA][CT]^CG
BspEI 758 TCCGGA T^CCGGA
BsrBI 376 CCGCTC CCGCTC(-3/-3)
BsrFI 1568 GCCGGC [GA]^CCGG[CT]
BssHII 150 GCGCGC G^CGCGC
BssSI 952 CACGAG CACGAG(-5/-1)
BstAPI 454 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 649 CCTGAGG CC^T[ACGT]AGG
CelII 942 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1568 GCCGGC [GA]^CCGG[CT]
Eco81I 649 CCTGAGG CC^T[ACGT]AGG
HincII 341 GTCAAC GT[CT]^[GA]AC
HindII 341 GTCAAC GT[CT]^[GA]AC
KasI 290 GGCGCC G^GCGCC
KpnI 403 GGTACC GGTAC^C
MroI 758 TCCGGA T^CCGGA
MslI 729 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1568 GCCGGC GCC^GGC
NarI 290 GGCGCC GG^CGCC
NgoMIV 1568 GCCGGC G^CCGGC
PshAI 1489 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 631 CCTGCAGG CCTGCA^GG
SfoI 290 GGCGCC GGC^GCC
SmlI 79 CTCAAG C^T[CT][GA]AG
Sse8387I 631 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 290 GGCGCC G[GA]^CG[CT]C
1299 GACGCC
AvaI 92 CCCGAG C^[CT]CG[GA]G
1052 CCCGAG
BalI 266 TGGCCA TGG^CCA
553 TGGCCA
BanI 290 GGCGCC G^G[CT][GA]CC
403 GGTACC
BbvCI 1431 CCTCAGC CCTCAGC(-5/-2)
1558 GCTGAGG
BsaHI 290 GGCGCC G[GA]^CG[CT]C
1299 GACGCC
BsgI 949 CTGCAC GTGCAG(16/14)
205 GTGCAG
BsmBI 363 CGTCTC CGTCTC(1/5)
1407 CGTCTC
BsoBI 92 CCCGAG C^[CT]CG[GA]G
1052 CCCGAG
BspMI 1316 ACCTGC ACCTGC(4/8)
679 GCAGGT
BsrDI 1328 CATTGC GCAATG(2/0)
425 GCAATG
DsaI 98 CCATGG C^C[GA][CT]GG
1338 CCATGG
HgaI 114 GACGC GACGC(5/10)
1299 GACGC
Hin1I 290 GGCGCC G[GA]^CG[CT]C
1299 GACGCC
MaeII 203 ACGT A^CGT
581 ACGT
MaeIII 790 GTCAC ^GT[ACGT]AC
1157 GTCAC
MluNI 266 TGGCCA TGG^CCA
553 TGGCCA
MscI 266 TGGCCA TGG^CCA
553 TGGCCA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
211 CAGCTG
NcoI 98 CCATGG C^CATGG
1338 CCATGG
PflMI 514 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1333 CCAGCCCATGG
PvuII 130 CAGCTG CAG^CTG
211 CAGCTG
SacI 955 GAGCTC GAGCT^C
1024 GAGCTC
TaqI 914 TCGA T^CGA
1472 TCGA
Tsp45I 790 GTCAC ^GT[GC]AC
1157 GTCAC
TthHB8I 914 TCGA T^CGA
1472 TCGA
Van91I 514 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1333 CCAGCCCATGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MboI MboII MflI
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI TspRI XhoII