Restriction map for
pF1KE0037
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AscI AseI AsnI AspI AviII
AvrII BalI BbeI BbrPI BciVI
BclI BfrI BglI BlnI BsaBI
BsmBI BsmI BspDI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KasI KspI
MamI MfeI MluI MluNI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 710 GGTACC G^GTACC
AccI 1009 GTCTAC GT^[AC][GT]AC
AcsI 1273 GAATTC [GA]^AATT[CT]
AhdI 265 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1117 GTGCAC G^TGCAC
Aor51HI 254 AGCGCT AGC^GCT
ApaLI 1117 GTGCAC G^TGCAC
ApoI 1273 GAATTC [GA]^AATT[CT]
Asp700I 691 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 710 GGTACC G^GTACC
AspEI 265 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 1214 GCAGATCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1815 AGATCT A^GATCT
BsiEI 287 CGGCCG CG[GA][CT]^CG
BsiWI 302 CGTACG C^GTACG
Bsp1407I 644 TGTACA T^GTACA
BsrBI 1419 CCGCTC CCGCTC(-3/-3)
BsrGI 644 TGTACA T^GTACA
EagI 287 CGGCCG C^GGCCG
Eam1105I 265 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 287 CGGCCG C^GGCCG
Eco47III 254 AGCGCT AGC^GCT
Eco52I 287 CGGCCG C^GGCCG
EcoNI 2096 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1273 GAATTC G^AATTC
FauI 1714 GCGGG CCCGC(4/6)
HaeII 254 AGCGCT [GA]GCGC^[CT]
HindIII 2132 AAGCTT A^AGCTT
KpnI 710 GGTACC GGTAC^C
MseI 521 TTAA T^TAA
NcoI 1571 CCATGG C^CATGG
SexAI 233 ACCTGGT A^CC[AT]GGT
SspBI 644 TGTACA T^GTACA
TaqI 573 TCGA T^CGA
TfiI 1725 GATTC G^A[AT]TC
Tru9I 521 TTAA T^TAA
TthHB8I 573 TCGA T^CGA
XcmI 1809 CCAGCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 691 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 507 CGCG CG^CG
805 CGCG
AccIII 826 TCCGGA T^CCGGA
1955 TCCGGA
AcyI 1205 GGCGTC G[GA]^CG[CT]C
1374 GACGCC
ApaI 360 GGGCCC GGGCC^C
1823 GGGCCC
BamHI 167 GGATCC G^GATCC
1460 GGATCC
BbsI 514 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BfaI 326 CTAG C^TAG
2006 CTAG
BlpI 675 GCTGAGC GC^T[ACGT]AGC
2157 GCTCAGC
Bpu1102I 675 GCTGAGC GC^T[ACGT]AGC
2157 GCTCAGC
BpuAI 514 GAAGAC GAAGAC(2/6)
1396 GTCTTC
BsaAI 300 CACGTA [CT]AC^GT[GA]
1084 TACGTG
BsaHI 1205 GGCGTC G[GA]^CG[CT]C
1374 GACGCC
BsaI 136 GAGACC GGTCTC(1/5)
875 GAGACC
BseAI 826 TCCGGA T^CCGGA
1955 TCCGGA
BsgI 238 GTGCAG GTGCAG(16/14)
1366 GTGCAG
BspEI 826 TCCGGA T^CCGGA
1955 TCCGGA
BsrDI 561 CATTGC GCAATG(2/0)
1578 CATTGC
BstAPI 240 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1888 GCAGAAGCTGC
BstUI 507 CGCG CG^CG
805 CGCG
CelII 675 GCTGAGC GC^T[ACGT]AGC
2157 GCTCAGC
EaeI 69 CGGCCA [CT]^GGCC[GA]
287 CGGCCG
Hin1I 1205 GGCGTC G[GA]^CG[CT]C
1374 GACGCC
MaeI 326 CTAG C^TAG
2006 CTAG
MroI 826 TCCGGA T^CCGGA
1955 TCCGGA
MvnI 507 CGCG CG^CG
805 CGCG
SacI 678 GAGCTC GAGCT^C
1162 GAGCTC
SbfI 1797 CCTGCAGG CCTGCA^GG
1947 CCTGCAGG
SmaI 1677 CCCGGG CCC^GGG
2079 CCCGGG
Sse8387I 1797 CCTGCAGG CCTGCA^GG
1947 CCTGCAGG
Tsp509I 840 AATT ^AATT
1274 AATT
XmaCI 1677 CCCGGG C^CCGGG
2079 CCCGGG
XmaI 1677 CCCGGG C^CCGGG
2079 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BpmI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TseI Tsp45I TspRI Van91I
XhoII