Restriction map for
pF1KB8882
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 49 GAATTT [GA]^AATT[CT]
AfaI 45 GTAC GT^AC
AlwNI 313 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 80 AGCGCT AGC^GCT
ApoI 49 GAATTT [GA]^AATT[CT]
AvaII 321 GGACC G^G[AT]CC
BanI 305 GGCACC G^G[CT][GA]CC
BanII 541 GAGCCC G[GA]GC[CT]^C
BcgI 343 CGAGAGTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 25 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 541 GAGCCC G[AGT]GC[ACT]^C
BpmI 289 CTGGAG CTGGAG(16/14)
BsmFI 339 GTCCC GGGAC(10/14)
Bsp1286I 541 GAGCCC G[AGT]GC[ACT]^C
BspMI 284 ACCTGC ACCTGC(4/8)
BsrBI 9 CCGCTC CCGCTC(-3/-3)
BstF5I 234 GGATG GGATG(2/0)
BstYI 110 AGATCC [GA]^GATC[CT]
Cac8I 369 GCCTGC GC[ACGT]^[ACGT]GC
DraII 187 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 460 GACACAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 80 AGCGCT AGC^GCT
EcoNI 285 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 187 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 132 CCATGG C^C[AT][AT]GG
FokI 234 GGATG GGATG(9/13)
HincII 379 GTCGAC GT[CT]^[GA]AC
HindII 379 GTCGAC GT[CT]^[GA]AC
HinfI 392 GAGTC G^A[ACGT]TC
MaeIII 262 GTGAC ^GT[ACGT]AC
MflI 110 AGATCC [GA]^GATC[CT]
MseI 424 TTAA T^TAA
MspA1I 164 CAGCTG C[AC]G^C[GT]G
NcoI 132 CCATGG C^CATGG
NspI 21 ACATGC [GA]CATG^[CT]
PflMI 312 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 392 GAGTC GAGTC(4/5)
PvuII 164 CAGCTG CAG^CTG
RsaI 45 GTAC GT^AC
SalI 379 GTCGAC G^TCGAC
SfaNI 419 GCATC GCATC(5/9)
StuI 93 AGGCCT AGG^CCT
StyI 132 CCATGG C^C[AT][AT]GG
TaqI 380 TCGA T^CGA
Tru9I 424 TTAA T^TAA
Tsp45I 262 GTGAC ^GT[GC]AC
Tsp509I 50 AATT ^AATT
TthHB8I 380 TCGA T^CGA
Van91I 312 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 110 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 379 GTCGAC GT^[AC][GT]AC
478 GTCTAC
AlwI 111 GATCC GGATC(4/5)
508 GATCC
BcnI 30 CCCGG CC^[GC]GG
302 CCCGG
BseRI 520 GAGGAG GAGGAG(10/8)
523 GAGGAG
DpnII 111 GATC ^GATC
508 GATC
DsaI 132 CCATGG C^C[GA][CT]GG
327 CCACGG
EaeI 32 CGGCCA [CT]^GGCC[GA]
447 TGGCCG
EarI 106 GAAGAG CTCTTC(1/4)
517 GAAGAG
HaeII 80 AGCGCT [GA]GCGC^[CT]
314 AGCGCC
HphI 73 GGTGA GGTGA(8/7)
261 GGTGA
Ksp632I 106 GAAGAG CTCTTC(1/4)
517 GAAGAG
MboI 111 GATC ^GATC
508 GATC
MboII 106 GAAGA GAAGA(8/7)
517 GAAGA
NciI 30 CCCGG CC^[GC]GG
302 CCCGG
NdeII 111 GATC ^GATC
508 GATC
NlaIV 187 GGGGCC GG[ACGT]^[ACGT]CC
305 GGCACC
Sau3AI 111 GATC ^GATC
508 GATC
TspRI 14 CAGTG CA[GC]TG[ACGT][ACGT]^
207 CAGTG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI AvaI BbvI BsaJI
BsiYI BslI BsoBI BssKI BstNI
CfoI Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MwoI
NlaIII Sau96I ScrFI TseI