Restriction map for
pF1KB6378
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI MaeI MaeIII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 844 GTAGAC GT^[AC][GT]AC
AccII 441 CGCG CG^CG
AfaI 428 GTAC GT^AC
ApaI 742 GGGCCC GGGCC^C
BlpI 825 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 825 GCTGAGC GC^T[ACGT]AGC
BsaI 503 GAGACC GGTCTC(1/5)
BspMI 614 ACCTGC ACCTGC(4/8)
BsrBI 4 CCGCTC CCGCTC(-3/-3)
BsrDI 673 GCAATG GCAATG(2/0)
BsrI 67 ACTGG ACTGG(1/-1)
BstUI 441 CGCG CG^CG
CelII 825 GCTGAGC GC^T[ACGT]AGC
EarI 33 GAAGAG CTCTTC(1/4)
EcoNI 659 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 580 GTTGAC GT[CT]^[GA]AC
HindII 580 GTTGAC GT[CT]^[GA]AC
HinfI 311 GATTC G^A[ACGT]TC
HphI 487 GGTGA GGTGA(8/7)
Ksp632I 33 GAAGAG CTCTTC(1/4)
KspI 440 CCGCGG CCGC^GG
MaeII 854 ACGT A^CGT
MboII 33 GAAGA GAAGA(8/7)
MslI 223 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 441 CGCG CG^CG
RsaI 428 GTAC GT^AC
SacII 440 CCGCGG CCGC^GG
SfcI 842 CTGTAG C^T[GA][CT]AG
SfiI 621 GGCCTATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 807 TCGA T^CGA
TfiI 311 GATTC G^A[AT]TC
TthHB8I 807 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 252 GGATC GGATC(4/5)
496 GGATC
AspHI 84 GAGCTC G[AT]GC[AT]^C
828 GAGCTC
AvaII 436 GGTCC G^G[AT]CC
765 GGTCC
BclI 53 TGATCA T^GATCA
387 TGATCA
BcnI 18 CCCGG CC^[GC]GG
162 CCGGG
BglI 446 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
622 GCCTATGGGGC
BpmI 68 CTGGAG CTGGAG(16/14)
416 CTGGAG
BsiHKAI 84 GAGCTC G[AT]GC[AT]^C
828 GAGCTC
BsmAI 490 GAGAC GTCTC(1/5)
503 GAGAC
DraII 741 GGGGCCC [GA]G^G[ACGT]CC[CT]
742 GGGCCCT
EcoO109I 741 GGGGCCC [GA]G^G[ACGT]CC[CT]
742 GGGCCCT
FauI 439 CCCGC CCCGC(4/6)
442 GCGGG
FbaI 53 TGATCA T^GATCA
387 TGATCA
NciI 18 CCCGG CC^[GC]GG
162 CCGGG
NcoI 559 CCATGG C^CATGG
572 CCATGG
PvuII 289 CAGCTG CAG^CTG
299 CAGCTG
SacI 84 GAGCTC GAGCT^C
828 GAGCTC
SfaNI 723 GATGC GCATC(5/9)
866 GCATC
StuI 698 AGGCCT AGG^CCT
793 AGGCCT
XcmI 399 CCATCTGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
728 CCAAGTTTGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HpaII ItaI MboI MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TseI TspRI