Restriction map for
pF1KB6417
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII Eam1105I
EarI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 439 GTAGAC GT^[AC][GT]AC
AcsI 751 AAATTT [GA]^AATT[CT]
AocI 840 CCTGAGG CC^T[ACGT]AGG
ApoI 751 AAATTT [GA]^AATT[CT]
Asp700I 822 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 667 GAGCTC G[AT]GC[AT]^C
AvaI 307 CTCGAG C^[CT]CG[GA]G
BanII 667 GAGCTC G[GA]GC[CT]^C
BbvCI 16 GCTGAGG CCTCAGC(-5/-2)
BfaI 662 CTAG C^TAG
BsaBI 795 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 78 GGTCTC GGTCTC(1/5)
BsiHKAI 667 GAGCTC G[AT]GC[AT]^C
BsoBI 307 CTCGAG C^[CT]CG[GA]G
BspMI 719 ACCTGC ACCTGC(4/8)
BsrDI 814 GCAATG GCAATG(2/0)
BstYI 218 AGATCC [GA]^GATC[CT]
Bsu36I 840 CCTGAGG CC^T[ACGT]AGG
EagI 170 CGGCCG C^GGCCG
EclXI 170 CGGCCG C^GGCCG
Eco52I 170 CGGCCG C^GGCCG
Eco81I 840 CCTGAGG CC^T[ACGT]AGG
EcoNI 903 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HinfI 897 GACTC G^A[ACGT]TC
HphI 479 TCACC GGTGA(8/7)
MaeI 662 CTAG C^TAG
MaeIII 737 GTGAC ^GT[ACGT]AC
MamI 795 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 819 GAAGA GAAGA(8/7)
MflI 218 AGATCC [GA]^GATC[CT]
MslI 117 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 434 CCGCTG C[AC]G^C[GT]G
NcoI 38 CCATGG C^CATGG
NdeI 505 CATATG CA^TATG
NspI 116 ACATGC [GA]CATG^[CT]
PaeR7I 307 CTCGAG C^TCGAG
PflMI 881 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 897 GACTC GAGTC(4/5)
SacI 667 GAGCTC GAGCT^C
ScaI 209 AGTACT AGT^ACT
SmlI 307 CTCGAG C^T[CT][GA]AG
StuI 911 AGGCCT AGG^CCT
Tsp45I 737 GTGAC ^GT[GC]AC
Tsp509I 752 AATT ^AATT
Van91I 881 CCAAATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 307 CTCGAG C^TCGAG
XhoII 218 AGATCC [GA]^GATC[CT]
XmnI 822 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 210 GTAC GT^AC
807 GTAC
BbeI 128 GGCGCC GGCGC^C
286 GGCGCC
BcnI 55 CCCGG CC^[GC]GG
75 CCCGG
BmyI 667 GAGCTC G[AGT]GC[ACT]^C
776 GTGCCC
BsgI 876 CTGCAC GTGCAG(16/14)
562 GTGCAG
BsiEI 9 CGACCG CG[GA][CT]^CG
170 CGGCCG
Bsp1286I 667 GAGCTC G[AGT]GC[ACT]^C
776 GTGCCC
DraII 51 AGGTCCC [GA]G^G[ACGT]CC[CT]
354 GGGACCC
DrdI 442 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
622 GACCGCGATGTC
DsaI 38 CCATGG C^C[GA][CT]GG
454 CCACGG
EaeI 170 CGGCCG [CT]^GGCC[GA]
431 TGGCCG
EcoO109I 51 AGGTCCC [GA]G^G[ACGT]CC[CT]
354 GGGACCC
EcoT14I 38 CCATGG C^C[AT][AT]GG
908 CCAAGG
HaeII 128 GGCGCC [GA]GCGC^[CT]
286 GGCGCC
HgaI 265 GACGC GACGC(5/10)
278 GACGC
KasI 128 GGCGCC G^GCGCC
286 GGCGCC
MaeII 152 ACGT A^CGT
338 ACGT
NarI 128 GGCGCC GG^CGCC
286 GGCGCC
NciI 55 CCCGG CC^[GC]GG
75 CCCGG
NlaIII 39 CATG CATG^
117 CATG
PpuMI 51 AGGTCCC [GA]G^G[AT]CC[CT]
354 GGGACCC
PstI 44 CTGCAG CTGCA^G
721 CTGCAG
RsaI 210 GTAC GT^AC
807 GTAC
SfoI 128 GGCGCC GGC^GCC
286 GGCGCC
StyI 38 CCATGG C^C[AT][AT]GG
908 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanI BbvI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HpaII ItaI MboI MnlI MspI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI TspRI TthHB8I