Restriction map for
pF1KE0518
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvrII BamHI
BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HgaI Hin1I
HindIII HpaI KasI KpnI Ksp632I
MamI MboII MfeI MflI MluI
MroI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 47 ACACGT A^C[GA][CT]GT
AvaII 371 GGACC G^G[AT]CC
AviII 458 TGCGCA TGC^GCA
BanI 195 GGTGCC G^G[CT][GA]CC
BbrPI 48 CACGTG CAC^GTG
BfaI 268 CTAG C^TAG
BglI 271 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 126 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 126 GCTGAGC GC^T[ACGT]AGC
BseRI 492 CTCCTC GAGGAG(10/8)
BsmFI 44 GGGAC GGGAC(10/14)
BsrBI 96 CCGCTC CCGCTC(-3/-3)
BstEII 228 GGTGACC G^GT[ACGT]ACC
BstPI 228 GGTGACC G^GT[ACGT]ACC
BstXI 74 CCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 126 GCTGAGC GC^T[ACGT]AGC
DdeI 127 CTGAG C^T[ACGT]AG
DpnII 34 GATC ^GATC
EcoNI 205 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 228 GGTGACC G^GT[ACGT]ACC
FspI 458 TGCGCA TGC^GCA
HaeII 220 AGCGCC [GA]GCGC^[CT]
HincII 393 GTTGAC GT[CT]^[GA]AC
HindII 393 GTTGAC GT[CT]^[GA]AC
HinfI 448 GACTC G^A[ACGT]TC
KspI 16 CCGCGG CCGC^GG
MaeI 268 CTAG C^TAG
MaeIII 229 GTGAC ^GT[ACGT]AC
MboI 34 GATC ^GATC
MslI 48 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 34 GATC ^GATC
PflMI 341 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 448 GACTC GAGTC(4/5)
PmaCI 48 CACGTG CAC^GTG
PmlI 48 CACGTG CAC^GTG
SacII 16 CCGCGG CCGC^GG
Sau3AI 34 GATC ^GATC
SfaNI 163 GATGC GCATC(5/9)
Tsp45I 229 GTGAC ^GT[GC]AC
Van91I 341 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 224 CCAGGGTGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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BalI 140 TGGCCA TGG^CCA
278 TGGCCA
BcnI 42 CCGGG CC^[GC]GG
286 CCGGG
BmyI 196 GTGCCC G[AGT]GC[ACT]^C
333 GGGCAC
BpmI 450 CTCCAG CTGGAG(16/14)
188 CTGGAG
BsaAI 48 CACGTG [CT]AC^GT[GA]
151 TACGTG
Bsp1286I 196 GTGCCC G[AGT]GC[ACT]^C
333 GGGCAC
BstF5I 399 CATCC GGATG(2/0)
162 GGATG
Cfr13I 350 GGCCC G^G[ACGT]CC
371 GGACC
DsaI 16 CCGCGG C^C[GA][CT]GG
233 CCGTGG
EaeI 140 TGGCCA [CT]^GGCC[GA]
278 TGGCCA
FauI 344 CCCGC CCCGC(4/6)
18 GCGGG
FokI 399 CATCC GGATG(9/13)
162 GGATG
HapII 42 CCGG C^CGG
286 CCGG
HpaII 42 CCGG C^CGG
286 CCGG
HphI 228 GGTGA GGTGA(8/7)
293 TCACC
MaeII 49 ACGT A^CGT
152 ACGT
MluNI 140 TGGCCA TGG^CCA
278 TGGCCA
MscI 140 TGGCCA TGG^CCA
278 TGGCCA
MspI 42 CCGG C^CGG
286 CCGG
NciI 42 CCGGG CC^[GC]GG
286 CCGGG
NlaIV 195 GGTGCC GG[ACGT]^[ACGT]CC
350 GGCCCC
Sau96I 350 GGCCC G^G[ACGT]CC
371 GGACC
TspRI 336 CACTG CA[GC]TG[ACGT][ACGT]^
476 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI EcoRII Fnu4HI HaeIII
HhaI HinP1I ItaI MnlI MspA1I
MvaI MvnI MwoI ScrFI TseI