Restriction map for
pF1KB7880
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BbeI
BbrPI BcgI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 1465 CTTAAG C^TTAAG
AlwNI 1425 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 12 AGCGCT AGC^GCT
AvaII 289 GGTCC G^G[AT]CC
AviII 252 TGCGCA TGC^GCA
BbsI 1609 GTCTTC GAAGAC(2/6)
BbvCI 220 GCTGAGG CCTCAGC(-5/-2)
BciVI 326 GTATCC GTATCC(6/5)
BclI 1215 TGATCA T^GATCA
BfrI 1465 CTTAAG C^TTAAG
BpuAI 1609 GTCTTC GAAGAC(2/6)
BsaBI 321 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 643 GAGACC GGTCTC(1/5)
BsaWI 1822 ACCGGA [AT]^CCGG[AT]
BsmAI 643 GAGAC GTCTC(1/5)
BsmFI 370 GGGAC GGGAC(10/14)
BspMI 437 ACCTGC ACCTGC(4/8)
BsrBI 1365 CCGCTC CCGCTC(-3/-3)
BstXI 456 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 1216 GATC ^GATC
DsaI 38 CCATGG C^C[GA][CT]GG
Eco47III 12 AGCGCT AGC^GCT
EcoT14I 38 CCATGG C^C[AT][AT]GG
FbaI 1215 TGATCA T^GATCA
FspI 252 TGCGCA TGC^GCA
HaeII 12 AGCGCT [GA]GCGC^[CT]
HapII 1823 CCGG C^CGG
HindIII 734 AAGCTT A^AGCTT
HpaII 1823 CCGG C^CGG
HphI 1218 TCACC GGTGA(8/7)
MamI 321 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1216 GATC ^GATC
MspI 1823 CCGG C^CGG
NcoI 38 CCATGG C^CATGG
NdeI 1344 CATATG CA^TATG
NdeII 1216 GATC ^GATC
PpuMI 288 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 939 GAGCTC GAGCT^C
SapI 1912 GAAGAGC GCTCTTC(1/4)
Sau3AI 1216 GATC ^GATC
StyI 38 CCATGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 404 TGGCCA TGG^CCA
1044 TGGCCA
BanII 939 GAGCTC G[GA]GC[CT]^C
1915 GAGCCC
BbvI 10 GCAGC GCAGC(8/12)
515 GCTGC
BspHI 156 TCATGA T^CATGA
335 TCATGA
BsrDI 1001 GCAATG GCAATG(2/0)
1592 GCAATG
BstF5I 297 CATCC GGATG(2/0)
579 CATCC
CfoI 13 GCGC GCG^C
253 GCGC
EarI 49 GAAGAG CTCTTC(1/4)
1912 GAAGAG
FokI 297 CATCC GGATG(9/13)
579 CATCC
HhaI 13 GCGC GCG^C
253 GCGC
HinP1I 13 GCGC G^CGC
253 GCGC
Ksp632I 49 GAAGAG CTCTTC(1/4)
1912 GAAGAG
MaeII 397 ACGT A^CGT
660 ACGT
MluNI 404 TGGCCA TGG^CCA
1044 TGGCCA
MscI 404 TGGCCA TGG^CCA
1044 TGGCCA
MseI 1466 TTAA T^TAA
1541 TTAA
MslI 310 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1114 CATTCCTATG
MwoI 4 GCCGAGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
220 GCTGAGGAGGC
NspI 1379 ACATGC [GA]CATG^[CT]
1765 GCATGT
RcaI 156 TCATGA T^CATGA
335 TCATGA
TaqI 920 TCGA T^CGA
1131 TCGA
Tru9I 1466 TTAA T^TAA
1541 TTAA
TseI 10 GCAGC G^C[AT]GC
515 GCTGC
TthHB8I 920 TCGA T^CGA
1131 TCGA
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI BanI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI DraII
EaeI EcoO109I EcoRI EcoRII Fnu4HI
HaeIII HinfI ItaI MaeI MaeIII
MboII MnlI MvaI NlaIII NlaIV
PleI RsaI Sau96I ScrFI SfaNI
SmlI StuI TfiI Tsp45I Tsp509I
TspRI XmnI