Restriction map for
pF1KB7766
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# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI ItaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MroI MspA1I MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TfiI TseI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 317 GTAGAC GT^[AC][GT]AC
AciI 1470 GCGG CCGC(-3/-1)
AfaI 647 GTAC GT^AC
AgeI 1385 ACCGGT A^CCGGT
AlwI 1052 GATCC GGATC(4/5)
Asp700I 510 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 155 TGATCA T^GATCA
BfaI 1487 CTAG C^TAG
BsaWI 1385 ACCGGT [AT]^CCGG[AT]
BseRI 46 GAGGAG GAGGAG(10/8)
BsmI 548 GCATTC GAATGC(1/-1)
BsrDI 54 GCAATG GCAATG(2/0)
BsrFI 1385 ACCGGT [GA]^CCGG[CT]
BstBI 504 TTCGAA TT^CGAA
BstF5I 360 GGATG GGATG(2/0)
Cfr10I 1385 ACCGGT [GA]^CCGG[CT]
EcoT14I 184 CCTTGG C^C[AT][AT]GG
FbaI 155 TGATCA T^GATCA
FokI 360 GGATG GGATG(9/13)
HapII 1386 CCGG C^CGG
HincII 9 GTTGAC GT[CT]^[GA]AC
HindII 9 GTTGAC GT[CT]^[GA]AC
HinfI 634 GAGTC G^A[ACGT]TC
HpaII 1386 CCGG C^CGG
HphI 158 TCACC GGTGA(8/7)
MaeI 1487 CTAG C^TAG
MaeIII 440 GTGAC ^GT[ACGT]AC
MslI 932 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1386 CCGG C^CGG
NspV 504 TTCGAA TT^CGAA
PinAI 1385 ACCGGT A^CCGGT
PleI 634 GAGTC GAGTC(4/5)
RsaI 647 GTAC GT^AC
SexAI 116 ACCTGGT A^CC[AT]GGT
SfaNI 372 GATGC GCATC(5/9)
SfuI 504 TTCGAA TT^CGAA
SspI 429 AATATT AAT^ATT
StyI 184 CCTTGG C^C[AT][AT]GG
TaqI 505 TCGA T^CGA
Tsp45I 440 GTGAC ^GT[GC]AC
TthHB8I 505 TCGA T^CGA
XmnI 510 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 968 GGTCC G^G[AT]CC
1388 GGTCC
BalI 26 TGGCCA TGG^CCA
256 TGGCCA
BbsI 195 GAAGAC GAAGAC(2/6)
121 GTCTTC
BpuAI 195 GAAGAC GAAGAC(2/6)
121 GTCTTC
BsaJI 126 CCTGGG C^C[ACGT][ACGT]GG
184 CCTTGG
BsmAI 300 GAGAC GTCTC(1/5)
139 GTCTC
BssKI 117 CCTGG ^CC[ACGT]GG
126 CCTGG
BstNI 117 CCTGG CC^[AT]GG
126 CCTGG
Cfr13I 968 GGTCC G^G[ACGT]CC
1388 GGTCC
DpnII 156 GATC ^GATC
1052 GATC
EaeI 26 TGGCCA [CT]^GGCC[GA]
256 TGGCCA
EcoRII 117 CCTGG ^CC[AT]GG
126 CCTGG
HaeIII 27 GGCC GG^CC
257 GGCC
MboI 156 GATC ^GATC
1052 GATC
MluNI 26 TGGCCA TGG^CCA
256 TGGCCA
MscI 26 TGGCCA TGG^CCA
256 TGGCCA
MvaI 117 CCTGG CC^[AT]GG
126 CCTGG
NdeII 156 GATC ^GATC
1052 GATC
NlaIII 202 CATG CATG^
322 CATG
Sau3AI 156 GATC ^GATC
1052 GATC
Sau96I 968 GGTCC G^G[ACGT]CC
1388 GGTCC
ScrFI 117 CCTGG CC^[ACGT]GG
126 CCTGG
SfcI 108 CTACAG C^T[GA][CT]AG
1172 CTGTAG
TspRI 403 CAGTG CA[GC]TG[ACGT][ACGT]^
436 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsiYI
BslI BsrI DdeI EcoRI HindIII
MboII MnlI MseI Tru9I Tsp509I