Restriction map for
pF1KB7954
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeII MamI MboI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NcoI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 37 GCGG CCGC(-3/-1)
AflII 271 CTTAAG C^TTAAG
AflIII 302 ACATGT A^C[GA][CT]GT
AspHI 86 GAGCTC G[AT]GC[AT]^C
BalI 737 TGGCCA TGG^CCA
BanI 775 GGTGCC G^G[CT][GA]CC
BanII 86 GAGCTC G[GA]GC[CT]^C
BbsI 1160 GTCTTC GAAGAC(2/6)
BcnI 1011 CCGGG CC^[GC]GG
BfaI 130 CTAG C^TAG
BfrI 271 CTTAAG C^TTAAG
BmyI 86 GAGCTC G[AGT]GC[ACT]^C
BpuAI 1160 GTCTTC GAAGAC(2/6)
BsiHKAI 86 GAGCTC G[AT]GC[AT]^C
BsiYI 865 CCAACCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 865 CCAACCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 984 GAGAC GTCTC(1/5)
BsmFI 3 GTCCC GGGAC(10/14)
Bsp1286I 86 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 387 TGTACA T^GTACA
BspLU11I 302 ACATGT A^CATGT
BspMI 46 GCAGGT ACCTGC(4/8)
BsrDI 942 CATTGC GCAATG(2/0)
BsrGI 387 TGTACA T^GTACA
BssSI 41 CACGAG CACGAG(-5/-1)
EaeI 737 TGGCCA [CT]^GGCC[GA]
EcoRI 836 GAATTC G^AATTC
EcoT14I 608 CCAAGG C^C[AT][AT]GG
HapII 1011 CCGG C^CGG
HindIII 25 AAGCTT A^AGCTT
HpaII 1011 CCGG C^CGG
HphI 570 GGTGA GGTGA(8/7)
MaeI 130 CTAG C^TAG
MluNI 737 TGGCCA TGG^CCA
MscI 737 TGGCCA TGG^CCA
MslI 888 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1057 CAGCTG C[AC]G^C[GT]G
MspI 1011 CCGG C^CGG
NciI 1011 CCGGG CC^[GC]GG
NdeI 226 CATATG CA^TATG
NlaIV 775 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 865 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1057 CAGCTG CAG^CTG
SacI 86 GAGCTC GAGCT^C
SapI 1045 GCTCTTC GCTCTTC(1/4)
SmlI 271 CTTAAG C^T[CT][GA]AG
SspBI 387 TGTACA T^GTACA
StuI 1127 AGGCCT AGG^CCT
StyI 608 CCAAGG C^C[AT][AT]GG
Van91I 865 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 197 CCAGAACCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 233 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
761 CAGAATCTG
Asp700I 650 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1042 GAAGCTCTTC
AvaII 210 GGACC G^G[AT]CC
556 GGACC
BpmI 728 CTCCAG CTGGAG(16/14)
560 CTGGAG
BsaJI 5 CCCTGG C^C[ACGT][ACGT]GG
608 CCAAGG
BstNI 6 CCTGG CC^[AT]GG
559 CCTGG
Cac8I 174 GCGAGC GC[ACGT]^[ACGT]GC
250 GCCAGC
EcoRII 6 CCTGG ^CC[AT]GG
559 CCTGG
MvaI 6 CCTGG CC^[AT]GG
559 CCTGG
NspI 302 ACATGT [GA]CATG^[CT]
528 ACATGC
PleI 1155 GACTC GAGTC(4/5)
507 GAGTC
PstI 758 CTGCAG CTGCA^G
1022 CTGCAG
SfaNI 193 GATGC GCATC(5/9)
697 GCATC
TfiI 763 GAATC G^A[AT]TC
1018 GAATC
Tsp45I 520 GTGAC ^GT[GC]AC
895 GTGAC
TspRI 516 CACTG CA[GC]TG[ACGT][ACGT]^
870 CAGTG
XmnI 650 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1042 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BsrI BssKI BstF5I Cfr13I DdeI
EarI Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeIII MboII MnlI
MseI MwoI NlaIII RsaI Sau96I
ScrFI SfcI TaqI Tru9I TseI
Tsp509I TthHB8I