##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MamI        MboII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NarI        NciI        
NcoI        NdeI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfcI        
SfiI        SfoI        SfuI        SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI           23  CCGC                 CCGC(-3/-1)
AcyI          156  GGCGTC               G[GA]^CG[CT]C
AlwNI         171  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BamHI           3  GGATCC               G^GATCC
BglI          110  GCCCTGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          131  GTGCCC               G[AGT]GC[ACT]^C
BsaHI         156  GGCGTC               G[GA]^CG[CT]C
Bsp1286I      131  GTGCCC               G[AGT]GC[ACT]^C
BspMI          40  ACCTGC               ACCTGC(4/8)
BssKI         135  CCAGG                ^CC[ACGT]GG
BstNI         135  CCAGG                CC^[AT]GG
BstYI           3  GGATCC               [GA]^GATC[CT]
Cac8I          46  GCCGGC               GC[ACGT]^[ACGT]GC
DpnII           4  GATC                 ^GATC
EcoRII        135  CCAGG                ^CC[AT]GG
HgaI          157  GCGTC                GACGC(5/10)
Hin1I         156  GGCGTC               G[GA]^CG[CT]C
HinfI          31  GACTC                G^A[ACGT]TC
HphI           28  GGTGA                GGTGA(8/7)
MaeIII         29  GTGAC                ^GT[ACGT]AC
MboI            4  GATC                 ^GATC
MflI            3  GGATCC               [GA]^GATC[CT]
MnlI           80  CCTC                 CCTC(7/6)
MspA1I        171  CAGCTG               C[AC]G^C[GT]G
MvaI          135  CCAGG                CC^[AT]GG
NaeI           46  GCCGGC               GCC^GGC
NdeII           4  GATC                 ^GATC
NgoMIV         46  GCCGGC               G^CCGGC
PleI           31  GACTC                GAGTC(4/5)
PvuII         171  CAGCTG               CAG^CTG
Sau3AI          4  GATC                 ^GATC
ScrFI         135  CCAGG                CC^[ACGT]GG
SfaNI         143  GCATC                GCATC(5/9)
SgrAI          24  CGCCGGTG             C[GA]^CCGG[CT]G
Tsp45I         29  GTGAC                ^GT[GC]AC
XhoII           3  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AluI           94  AGCT                 AG^CT
              172  AGCT                
AlwI            4  GATCC                GGATC(4/5)
                3  GGATC               
BsaJI         134  CCCAGG               C^C[ACGT][ACGT]GG
              135  CCAGGG              
BsgI           36  CTGCAC               GTGCAG(16/14)
              168  GTGCAG              
BsrFI          25  GCCGGT               [GA]^CCGG[CT]
               46  GCCGGC              
Cfr10I         25  GCCGGT               [GA]^CCGG[CT]
               46  GCCGGC              
HapII          26  CCGG                 C^CGG
               47  CCGG                
HpaII          26  CCGG                 C^CGG
               47  CCGG                
MspI           26  CCGG                 C^CGG
               47  CCGG                
NlaIV           3  GGATCC               GG[ACGT]^[ACGT]CC
               49  GGCTCC              


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

BbvI        BstAPI      CfoI        Fnu4HI      HhaI        
HinP1I      ItaI        MwoI        TseI