##################################
# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MaeII MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NdeI NdeII NheI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 407 GGACC G^G[AT]CC
BsmBI 619 GAGACG CGTCTC(1/5)
BspMI 624 GCAGGT ACCTGC(4/8)
BsrBI 322 CCGCTC CCGCTC(-3/-3)
BsrI 267 ACTGG ACTGG(1/-1)
BstF5I 416 GGATG GGATG(2/0)
DraIII 14 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 309 CTCTTC CTCTTC(1/4)
FokI 416 GGATG GGATG(9/13)
Ksp632I 309 CTCTTC CTCTTC(1/4)
NcoI 107 CCATGG C^CATGG
NspI 875 GCATGT [GA]CATG^[CT]
PpuMI 406 AGGACCC [GA]G^G[AT]CC[CT]
SmlI 352 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 567 GTAC GT^AC
879 GTAC
AvaI 391 CCCGAG C^[CT]CG[GA]G
400 CCCGAG
BbeI 222 GGCGCC GGCGC^C
837 GGCGCC
BglI 85 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
444 GCCGGCCAGGC
BlpI 456 GCTGAGC GC^T[ACGT]AGC
606 GCTCAGC
Bpu1102I 456 GCTGAGC GC^T[ACGT]AGC
606 GCTCAGC
BseRI 349 CTCCTC GAGGAG(10/8)
121 GAGGAG
BsgI 471 CTGCAC GTGCAG(16/14)
238 GTGCAG
BsiEI 319 CGGCCG CG[GA][CT]^CG
773 CGGCCG
BsmAI 619 GAGAC GTCTC(1/5)
666 GAGAC
BsoBI 391 CCCGAG C^[CT]CG[GA]G
400 CCCGAG
BssHII 585 GCGCGC G^CGCGC
819 GCGCGC
CelII 456 GCTGAGC GC^T[ACGT]AGC
606 GCTCAGC
DraII 74 AGGCCCC [GA]G^G[ACGT]CC[CT]
406 AGGACCC
EagI 319 CGGCCG C^GGCCG
773 CGGCCG
EclXI 319 CGGCCG C^GGCCG
773 CGGCCG
Eco52I 319 CGGCCG C^GGCCG
773 CGGCCG
EcoO109I 74 AGGCCCC [GA]G^G[ACGT]CC[CT]
406 AGGACCC
HphI 627 GGTGA GGTGA(8/7)
518 TCACC
KasI 222 GGCGCC G^GCGCC
837 GGCGCC
NaeI 51 GCCGGC GCC^GGC
444 GCCGGC
NarI 222 GGCGCC GG^CGCC
837 GGCGCC
NgoMIV 51 GCCGGC G^CCGGC
444 GCCGGC
NotI 318 GCGGCCGC GC^GGCCGC
772 GCGGCCGC
PvuII 529 CAGCTG CAG^CTG
764 CAGCTG
RsaI 567 GTAC GT^AC
879 GTAC
SfaNI 367 GATGC GCATC(5/9)
417 GATGC
SfcI 767 CTGTAG C^T[GA][CT]AG
870 CTACAG
SfoI 222 GGCGCC GGC^GCC
837 GGCGCC
TspRI 266 CACTG CA[GC]TG[ACGT][ACGT]^
615 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KspI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI SacII
Sau96I ScrFI StyI TseI