Restriction map for
pF1KE0027
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsaWI BseAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI StuI SwaI Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 142 GACGTC GACGT^C
AcyI 142 GACGTC G[GA]^CG[CT]C
AflIII 1225 ACACGT A^C[GA][CT]GT
Alw44I 862 GTGCAC G^TGCAC
ApaI 11 GGGCCC GGGCC^C
ApaLI 862 GTGCAC G^TGCAC
AspI 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1251 GCTGAGG CCTCAGC(-5/-2)
BglI 991 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1077 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1077 GCTGAGC GC^T[ACGT]AGC
BsaAI 652 TACGTG [CT]AC^GT[GA]
BsaHI 142 GACGTC G[GA]^CG[CT]C
BsiEI 104 CGACCG CG[GA][CT]^CG
BsiWI 154 CGTACG C^GTACG
BspHI 1382 TCATGA T^CATGA
BsrFI 49 GCCGGT [GA]^CCGG[CT]
BssHII 62 GCGCGC G^CGCGC
BstXI 1607 CCAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1077 GCTGAGC GC^T[ACGT]AGC
Cfr10I 49 GCCGGT [GA]^CCGG[CT]
DrdI 1191 GACCCCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 234 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1322 ATGCAT ATGCA^T
HaeII 1598 GGCGCT [GA]GCGC^[CT]
HgaI 165 GACGC GACGC(5/10)
Hin1I 142 GACGTC G[GA]^CG[CT]C
MseI 841 TTAA T^TAA
NsiI 1322 ATGCAT ATGCA^T
PflFI 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1382 TCATGA T^CATGA
SacI 228 GAGCTC GAGCT^C
SapI 625 GAAGAGC GCTCTTC(1/4)
SbfI 1412 CCTGCAGG CCTGCA^GG
SexAI 792 ACCTGGT A^CC[AT]GGT
SmlI 1446 CTCAAG C^T[CT][GA]AG
SphI 1270 GCATGC GCATG^C
Sse8387I 1412 CCTGCAGG CCTGCA^GG
SspI 755 AATATT AAT^ATT
TaqI 1589 TCGA T^CGA
Tru9I 841 TTAA T^TAA
Tth111I 1012 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1589 TCGA T^CGA
XcmI 1055 CCATGGTTCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 687 GATCC GGATC(4/5)
210 GGATC
AvaII 796 GGTCC G^G[AT]CC
1190 GGACC
BalI 585 TGGCCA TGG^CCA
1568 TGGCCA
BseRI 1424 CTCCTC GAGGAG(10/8)
1175 GAGGAG
BssSI 859 CTCGTG CACGAG(-5/-1)
1702 CTCGTG
BstYI 210 GGATCT [GA]^GATC[CT]
686 AGATCC
DpnII 211 GATC ^GATC
687 GATC
DraII 10 GGGGCCC [GA]G^G[ACGT]CC[CT]
782 GGGGCCT
DsaI 1055 CCATGG C^C[GA][CT]GG
1186 CCATGG
EcoO109I 10 GGGGCCC [GA]G^G[ACGT]CC[CT]
782 GGGGCCT
MboI 211 GATC ^GATC
687 GATC
MflI 210 GGATCT [GA]^GATC[CT]
686 AGATCC
MluNI 585 TGGCCA TGG^CCA
1568 TGGCCA
MscI 585 TGGCCA TGG^CCA
1568 TGGCCA
NcoI 1055 CCATGG C^CATGG
1186 CCATGG
NdeII 211 GATC ^GATC
687 GATC
Sau3AI 211 GATC ^GATC
687 GATC
SmaI 14 CCCGGG CCC^GGG
123 CCCGGG
XhoII 210 GGATCT [GA]^GATC[CT]
686 AGATCC
XmaCI 14 CCCGGG C^CCGGG
123 CCCGGG
XmaI 14 CCCGGG C^CCGGG
123 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
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AccII AciI AfaI AluI AlwNI
AspHI AvaI BanII BbvI BcnI
BmyI BpmI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MslI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
NspI PflMI PleI PstI RsaI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp45I TspRI Van91I