Restriction map for
pF1KB5983
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglII BlnI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SmlI SnaBI SpeI Sse8387I
SspBI SspI Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AfaI 90 GTAC GT^AC
AgeI 985 ACCGGT A^CCGGT
AocI 279 CCTGAGG CC^T[ACGT]AGG
AspHI 285 GAGCAC G[AT]GC[AT]^C
AvaI 1550 CCCGGG C^[CT]CG[GA]G
BbsI 1170 GAAGAC GAAGAC(2/6)
BbvCI 1102 CCTCAGC CCTCAGC(-5/-2)
BfaI 1217 CTAG C^TAG
BglI 1519 GCCTGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1018 GCTGAGC GC^T[ACGT]AGC
BpmI 550 CTGGAG CTGGAG(16/14)
Bpu1102I 1018 GCTGAGC GC^T[ACGT]AGC
BpuAI 1170 GAAGAC GAAGAC(2/6)
BsaBI 229 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 985 ACCGGT [AT]^CCGG[AT]
BseRI 282 GAGGAG GAGGAG(10/8)
BsgI 1156 CTGCAC GTGCAG(16/14)
BsiHKAI 285 GAGCAC G[AT]GC[AT]^C
BsmAI 1038 GTCTC GTCTC(1/5)
BsoBI 1550 CCCGGG C^[CT]CG[GA]G
BspMI 311 GCAGGT ACCTGC(4/8)
BsrDI 1031 CATTGC GCAATG(2/0)
BsrI 929 CCAGT ACTGG(1/-1)
BstEII 1321 GGTGACC G^GT[ACGT]ACC
BstPI 1321 GGTGACC G^GT[ACGT]ACC
BstXI 162 CCATTTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 143 AGATCC [GA]^GATC[CT]
Bsu36I 279 CCTGAGG CC^T[ACGT]AGG
CelII 1018 GCTGAGC GC^T[ACGT]AGC
DraI 25 TTTAAA TTT^AAA
DraII 1280 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 1150 CTCTTC CTCTTC(1/4)
Eco81I 279 CCTGAGG CC^T[ACGT]AGG
EcoNI 756 CCTTTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1280 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1321 GGTGACC G^GT[ACGT]ACC
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
HindIII 1294 AAGCTT A^AGCTT
Ksp632I 1150 CTCTTC CTCTTC(1/4)
MaeI 1217 CTAG C^TAG
MamI 229 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 143 AGATCC [GA]^GATC[CT]
MspA1I 547 CAGCTG C[AC]G^C[GT]G
NaeI 1115 GCCGGC GCC^GGC
NdeI 827 CATATG CA^TATG
NgoMIV 1115 GCCGGC G^CCGGC
NlaIV 908 GGAGCC GG[ACGT]^[ACGT]CC
PinAI 985 ACCGGT A^CCGGT
PpuMI 1280 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 309 CTGCAG CTGCA^G
PvuII 547 CAGCTG CAG^CTG
RsaI 90 GTAC GT^AC
SapI 1149 GCTCTTC GCTCTTC(1/4)
SfaNI 94 GATGC GCATC(5/9)
SgrAI 984 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1550 CCCGGG CCC^GGG
SphI 1139 GCATGC GCATG^C
SwaI 24 ATTTAAAT ATTT^AAAT
TaqI 59 TCGA T^CGA
TthHB8I 59 TCGA T^CGA
XhoII 143 AGATCC [GA]^GATC[CT]
XmaCI 1550 CCCGGG C^CCGGG
XmaI 1550 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 144 GATCC GGATC(4/5)
159 GATCC
AlwNI 180 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGTCTCTG
Aor51HI 305 AGCGCT AGC^GCT
1093 AGCGCT
AspI 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
855 GACTTCGTC
AvaII 1049 GGTCC G^G[AT]CC
1281 GGTCC
BcnI 1550 CCCGG CC^[GC]GG
1551 CCGGG
BmyI 285 GAGCAC G[AGT]GC[ACT]^C
1355 GGGCAC
Bsp1286I 285 GAGCAC G[AGT]GC[ACT]^C
1355 GGGCAC
BsrFI 985 ACCGGT [GA]^CCGG[CT]
1115 GCCGGC
Cfr10I 985 ACCGGT [GA]^CCGG[CT]
1115 GCCGGC
Cfr13I 1049 GGTCC G^G[ACGT]CC
1281 GGTCC
Eco47III 305 AGCGCT AGC^GCT
1093 AGCGCT
EcoT14I 74 CCAAGG C^C[AT][AT]GG
1398 CCTTGG
HaeIII 343 GGCC GG^CC
683 GGCC
MaeII 698 ACGT A^CGT
722 ACGT
MslI 163 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
717 CATGAACGTG
NciI 1550 CCCGG CC^[GC]GG
1551 CCGGG
NspI 708 ACATGC [GA]CATG^[CT]
1139 GCATGC
PflFI 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
855 GACTTCGTC
Sau96I 1049 GGTCC G^G[ACGT]CC
1281 GGTCC
StuI 342 AGGCCT AGG^CCT
682 AGGCCT
StyI 74 CCAAGG C^C[AT][AT]GG
1398 CCTTGG
TspRI 351 CACTG CA[GC]TG[ACGT][ACGT]^
1062 CAGTG
Tth111I 328 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
855 GACTTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
BbvI BsaJI BsiYI BslI BssKI
BstF5I BstNI BstUI Cac8I CfoI
DdeI DpnII EcoRII Fnu4HI FokI
HaeII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NdeII NlaIII PleI
Sau3AI ScrFI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I