Restriction map for
pF1KE0139
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
Bst1107I BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FokI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI NarI NcoI NdeI NdeII
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I TspRI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 433 GTCTAC GT^[AC][GT]AC
AccII 14 CGCG CG^CG
AcsI 139 GAATTC [GA]^AATT[CT]
AfaI 353 GTAC GT^AC
ApoI 139 GAATTC [GA]^AATT[CT]
AvaII 108 GGACC G^G[AT]CC
BcnI 158 CCCGG CC^[GC]GG
BpmI 166 CTGGAG CTGGAG(16/14)
BsaWI 149 ACCGGA [AT]^CCGG[AT]
BsgI 54 GTGCAG GTGCAG(16/14)
BsiEI 10 CGGTCG CG[GA][CT]^CG
BspMI 56 GCAGGT ACCTGC(4/8)
BsrBI 193 GAGCGG CCGCTC(-3/-3)
BsrFI 87 GCCGGC [GA]^CCGG[CT]
BssSI 320 CTCGTG CACGAG(-5/-1)
BstAPI 234 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 14 CGCG CG^CG
CfoI 4 GCGC GCG^C
Cfr10I 87 GCCGGC [GA]^CCGG[CT]
Cfr13I 108 GGACC G^G[ACGT]CC
DsaI 448 CCGTGG C^C[GA][CT]GG
EcoRI 139 GAATTC G^AATTC
HaeII 3 GGCGCT [GA]GCGC^[CT]
HaeIII 312 GGCC GG^CC
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HincII 72 GTCAAC GT[CT]^[GA]AC
HindII 72 GTCAAC GT[CT]^[GA]AC
HphI 143 TCACC GGTGA(8/7)
MseI 335 TTAA T^TAA
MvnI 14 CGCG CG^CG
NaeI 87 GCCGGC GCC^GGC
NciI 158 CCCGG CC^[GC]GG
NgoMIV 87 GCCGGC G^CCGGC
RsaI 353 GTAC GT^AC
Sau96I 108 GGACC G^G[ACGT]CC
SfcI 179 CTGTAG C^T[GA][CT]AG
TaqI 469 TCGA T^CGA
Tru9I 335 TTAA T^TAA
Tsp509I 140 AATT ^AATT
TthHB8I 469 TCGA T^CGA
XcmI 356 CCAGAAATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 7 enzymes) #
###################################
BsiYI 159 CCGGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
447 CCCGTGGGAGG
BslI 159 CCGGAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
447 CCCGTGGGAGG
BstXI 356 CCAGAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
442 CCAACCCCGTGG
FauI 195 GCGGG CCCGC(4/6)
415 GCGGG
MslI 286 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
398 CACAGAGATG
PleI 255 GACTC GAGTC(4/5)
466 GACTC
TfiI 419 GAATC G^A[AT]TC
530 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstNI Cac8I DdeI EcoRII Fnu4HI
HapII HinfI HpaII ItaI MnlI
MspI MvaI MwoI NlaIII NlaIV
ScrFI SfaNI TseI