Restriction map for
pF1KE0146
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
CfoI ClaI CpoI DdeI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KspI MaeII MfeI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 59 CGCG CG^CG
AcsI 682 AAATTT [GA]^AATT[CT]
AcyI 269 GACGCC G[GA]^CG[CT]C
AflII 871 CTTAAG C^TTAAG
ApoI 682 AAATTT [GA]^AATT[CT]
AvaI 29 CCCGGG C^[CT]CG[GA]G
BalI 71 TGGCCA TGG^CCA
BbsI 113 GAAGAC GAAGAC(2/6)
BbvI 493 GCTGC GCAGC(8/12)
BfrI 871 CTTAAG C^TTAAG
BpmI 809 CTCCAG CTGGAG(16/14)
BpuAI 113 GAAGAC GAAGAC(2/6)
BsaBI 859 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 269 GACGCC G[GA]^CG[CT]C
BsaWI 105 ACCGGA [AT]^CCGG[AT]
BseRI 424 GAGGAG GAGGAG(10/8)
BsmAI 290 GTCTC GTCTC(1/5)
BsmFI 8 GTCCC GGGAC(10/14)
BsmI 608 GAATGC GAATGC(1/-1)
BsoBI 29 CCCGGG C^[CT]CG[GA]G
BsrFI 4 GCCGGT [GA]^CCGG[CT]
BstUI 59 CGCG CG^CG
Cac8I 53 GCCTGC GC[ACGT]^[ACGT]GC
Cfr10I 4 GCCGGT [GA]^CCGG[CT]
DrdI 116 GACCAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 657 CCGTGG C^C[GA][CT]GG
EcoRV 736 GATATC GAT^ATC
EcoT14I 784 CCAAGG C^C[AT][AT]GG
FauI 188 CCCGC CCCGC(4/6)
HgaI 269 GACGC GACGC(5/10)
Hin1I 269 GACGCC G[GA]^CG[CT]C
HincII 338 GTCAAC GT[CT]^[GA]AC
HindII 338 GTCAAC GT[CT]^[GA]AC
HphI 822 GGTGA GGTGA(8/7)
MamI 859 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 71 TGGCCA TGG^CCA
MscI 71 TGGCCA TGG^CCA
MspA1I 415 CAGCTG C[AC]G^C[GT]G
MvnI 59 CGCG CG^CG
PstI 301 CTGCAG CTGCA^G
PvuII 415 CAGCTG CAG^CTG
SfaNI 646 GATGC GCATC(5/9)
SmaI 29 CCCGGG CCC^GGG
SmlI 871 CTTAAG C^T[CT][GA]AG
SspI 457 AATATT AAT^ATT
StyI 784 CCAAGG C^C[AT][AT]GG
TseI 493 GCTGC G^C[AT]GC
Tsp45I 379 GTGAC ^GT[GC]AC
Tsp509I 683 AATT ^AATT
TspRI 642 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 357 CCAGCTTGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 630 GGTACC G^GTACC
662 GGTACC
ApaI 26 GGGCCC GGGCC^C
32 GGGCCC
Asp718I 630 GGTACC G^GTACC
662 GGTACC
AvaII 7 GGTCC G^G[AT]CC
39 GGACC
BamHI 672 GGATCC G^GATCC
780 GGATCC
BanI 630 GGTACC G^G[CT][GA]CC
662 GGTACC
BanII 26 GGGCCC G[GA]GC[CT]^C
32 GGGCCC
BmyI 26 GGGCCC G[AGT]GC[ACT]^C
32 GGGCCC
Bsp1286I 26 GGGCCC G[AGT]GC[ACT]^C
32 GGGCCC
BsrI 627 ACTGG ACTGG(1/-1)
273 CCAGT
BstYI 672 GGATCC [GA]^GATC[CT]
780 GGATCC
EarI 754 CTCTTC CTCTTC(1/4)
131 GAAGAG
Fnu4HI 57 GCCGC GC^[ACGT]GC
493 GCTGC
ItaI 57 GCCGC GC^[ACGT]GC
493 GCTGC
KpnI 630 GGTACC GGTAC^C
662 GGTACC
Ksp632I 754 CTCTTC CTCTTC(1/4)
131 GAAGAG
MaeIII 379 GTGAC ^GT[ACGT]AC
520 GTTAC
MflI 672 GGATCC [GA]^GATC[CT]
780 GGATCC
MwoI 487 GCTATTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
670 GCGGATCCTGC
PleI 213 GACTC GAGTC(4/5)
288 GAGTC
TfiI 87 GAATC G^A[AT]TC
230 GAATC
XhoII 672 GGATCC [GA]^GATC[CT]
780 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI BcnI
BfaI BsaJI BsiYI BslI BssKI
BstF5I BstNI Cfr13I DpnII DraII
EaeI EcoO109I EcoRII FokI HaeIII
HapII HinfI HpaII MaeI MboI
MboII MnlI MseI MspI MvaI
NciI NdeII NlaIV RsaI Sau3AI
Sau96I ScrFI SfcI Tru9I