Restriction map for
pF1KB7926
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FseI FspI HgaI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI NcoI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 12 CGCG CG^CG
AcsI 868 AAATTC [GA]^AATT[CT]
AcyI 135 GGCGCC G[GA]^CG[CT]C
AflIII 858 ACATGT A^C[GA][CT]GT
AlwI 895 GATCC GGATC(4/5)
ApoI 868 AAATTC [GA]^AATT[CT]
AseI 367 ATTAAT AT^TAAT
AsnI 367 ATTAAT AT^TAAT
Asp700I 445 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 135 GGCGCC G^G[CT][GA]CC
BanII 155 GGGCTC G[GA]GC[CT]^C
BbeI 135 GGCGCC GGCGC^C
BbsI 449 GTCTTC GAAGAC(2/6)
BcgI 51 CGAGGGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 706 AGATCT A^GATCT
BmyI 155 GGGCTC G[AGT]GC[ACT]^C
BpuAI 449 GTCTTC GAAGAC(2/6)
BsaHI 135 GGCGCC G[GA]^CG[CT]C
BsgI 107 CTGCAC GTGCAG(16/14)
BsmBI 148 GAGACG CGTCTC(1/5)
Bsp1286I 155 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 584 TGTACA T^GTACA
BspLU11I 858 ACATGT A^CATGT
BspMI 669 ACCTGC ACCTGC(4/8)
BsrDI 988 GCAATG GCAATG(2/0)
BsrGI 584 TGTACA T^GTACA
BstF5I 996 GGATG GGATG(2/0)
BstNI 207 CCTGG CC^[AT]GG
BstUI 12 CGCG CG^CG
BstYI 706 AGATCT [GA]^GATC[CT]
EcoRII 207 CCTGG ^CC[AT]GG
EcoT22I 628 ATGCAT ATGCA^T
FokI 996 GGATG GGATG(9/13)
Hin1I 135 GGCGCC G[GA]^CG[CT]C
KasI 135 GGCGCC G^GCGCC
MaeII 969 ACGT A^CGT
MflI 706 AGATCT [GA]^GATC[CT]
MspA1I 116 CAGCGG C[AC]G^C[GT]G
MvaI 207 CCTGG CC^[AT]GG
MvnI 12 CGCG CG^CG
NaeI 332 GCCGGC GCC^GGC
NarI 135 GGCGCC GG^CGCC
NgoMIV 332 GCCGGC G^CCGGC
NsiI 628 ATGCAT ATGCA^T
NspI 858 ACATGT [GA]CATG^[CT]
PshAI 298 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 367 ATTAAT AT^TAAT
PstI 59 CTGCAG CTGCA^G
SfoI 135 GGCGCC GGC^GCC
SspBI 584 TGTACA T^GTACA
TfiI 724 GAATC G^A[AT]TC
TspRI 728 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 706 AGATCT [GA]^GATC[CT]
XmnI 445 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaII 130 GGACC G^G[AT]CC
561 GGACC
BclI 596 TGATCA T^GATCA
927 TGATCA
BcnI 160 CCCGG CC^[GC]GG
176 CCCGG
BsaJI 50 CCGAGG C^C[ACGT][ACGT]GG
207 CCTGGG
BsmAI 148 GAGAC GTCTC(1/5)
85 GTCTC
BsrFI 132 ACCGGC [GA]^CCGG[CT]
332 GCCGGC
BsrI 768 ACTGG ACTGG(1/-1)
685 CCAGT
Cfr10I 132 ACCGGC [GA]^CCGG[CT]
332 GCCGGC
EarI 259 GAAGAG CTCTTC(1/4)
280 GAAGAG
FbaI 596 TGATCA T^GATCA
927 TGATCA
Ksp632I 259 GAAGAG CTCTTC(1/4)
280 GAAGAG
NciI 160 CCCGG CC^[GC]GG
176 CCCGG
PleI 340 GACTC GAGTC(4/5)
303 GAGTC
SfcI 59 CTGCAG C^T[GA][CT]AG
492 CTACAG
TaqI 230 TCGA T^CGA
831 TCGA
Tsp45I 19 GTGAC ^GT[GC]AC
305 GTCAC
Tsp509I 590 AATT ^AATT
869 AATT
TthHB8I 230 TCGA T^CGA
831 TCGA
XbaI 603 TCTAGA T^CTAGA
654 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BpmI BseRI BsiYI BslI BsmFI
BssKI Cac8I CfoI Cfr13I DdeI
DpnII FauI Fnu4HI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI Tru9I TseI