Restriction map for
pF1KE0401
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII HphI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Asp700I 334 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 9 GGATCC G^GATCC
BbvI 261 GCAGC GCAGC(8/12)
BciVI 535 GTATCC GTATCC(6/5)
BfaI 512 CTAG C^TAG
BsaAI 272 TACGTA [CT]AC^GT[GA]
BsaJI 539 CCCTGG C^C[ACGT][ACGT]GG
BsgI 172 GTGCAG GTGCAG(16/14)
BsmFI 508 GGGAC GGGAC(10/14)
BspHI 209 TCATGA T^CATGA
BsrDI 75 CATTGC GCAATG(2/0)
BssKI 540 CCTGG ^CC[ACGT]GG
BstNI 540 CCTGG CC^[AT]GG
BstXI 30 CCAGTTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 401 CTAAG C^T[ACGT]AG
DraII 407 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 427 GAAGAG CTCTTC(1/4)
EcoO109I 407 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 540 CCTGG ^CC[AT]GG
Fnu4HI 261 GCAGC GC^[ACGT]GC
HincII 465 GTTGAC GT[CT]^[GA]AC
HindII 465 GTTGAC GT[CT]^[GA]AC
ItaI 261 GCAGC GC^[ACGT]GC
Ksp632I 427 GAAGAG CTCTTC(1/4)
MaeI 512 CTAG C^TAG
MslI 50 CACAAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 262 CAGCTG C[AC]G^C[GT]G
MvaI 540 CCTGG CC^[AT]GG
MwoI 409 GCCCTTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 9 GGATCC GG[ACGT]^[ACGT]CC
NspI 59 GCATGC [GA]CATG^[CT]
PvuII 262 CAGCTG CAG^CTG
RcaI 209 TCATGA T^CATGA
SapI 427 GAAGAGC GCTCTTC(1/4)
ScrFI 540 CCTGG CC^[ACGT]GG
SfcI 562 CTACAG C^T[GA][CT]AG
SnaBI 272 TACGTA TAC^GTA
SpeI 511 ACTAGT A^CTAGT
SphI 59 GCATGC GCATG^C
TaqI 595 TCGA T^CGA
TseI 261 GCAGC G^C[AT]GC
Tsp45I 158 GTGAC ^GT[GC]AC
TspRI 170 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 595 TCGA T^CGA
XmnI 334 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 381 GTAC GT^AC
398 GTAC
BpmI 349 CTCCAG CTGGAG(16/14)
552 CTCCAG
BsmAI 316 GAGAC GTCTC(1/5)
575 GAGAC
BsrI 30 CCAGT ACTGG(1/-1)
169 CCAGT
BstF5I 147 CATCC GGATG(2/0)
141 GGATG
BstYI 9 GGATCC [GA]^GATC[CT]
68 GGATCT
Cfr13I 27 GGCCC G^G[ACGT]CC
408 GGCCC
FokI 147 CATCC GGATG(9/13)
141 GGATG
HinfI 177 GATTC G^A[ACGT]TC
592 GAATC
MaeIII 158 GTGAC ^GT[ACGT]AC
369 GTTAC
MflI 9 GGATCC [GA]^GATC[CT]
68 GGATCT
RsaI 381 GTAC GT^AC
398 GTAC
Sau96I 27 GGCCC G^G[ACGT]CC
408 GGCCC
TfiI 177 GATTC G^A[AT]TC
592 GAATC
XhoII 9 GGATCC [GA]^GATC[CT]
68 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI AlwI Cac8I DpnII HaeIII
MaeII MboI MboII MnlI NdeII
NlaIII Sau3AI SfaNI Tsp509I