Restriction map for
pF1KE0017
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AvaI AviII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglII BlpI Bpu1102I BsaAI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 1632 CGCG CG^CG
AclI 983 AACGTT AA^CGTT
AcsI 249 AAATTC [GA]^AATT[CT]
AcyI 381 GACGCC G[GA]^CG[CT]C
AfaI 576 GTAC GT^AC
AflIII 1406 ACATGT A^C[GA][CT]GT
AhdI 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 249 AAATTC [GA]^AATT[CT]
AspEI 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 87 GGTCC G^G[AT]CC
AvrII 421 CCTAGG C^CTAGG
BanII 27 GAGCCC G[GA]GC[CT]^C
BbvI 806 GCTGC GCAGC(8/12)
BclI 1146 TGATCA T^GATCA
BglI 1543 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 421 CCTAGG C^CTAGG
BpmI 1043 CTGGAG CTGGAG(16/14)
BsaBI 898 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 381 GACGCC G[GA]^CG[CT]C
BsgI 1410 GTGCAG GTGCAG(16/14)
BspHI 57 TCATGA T^CATGA
BspLU11I 1406 ACATGT A^CATGT
BsrFI 1127 ACCGGC [GA]^CCGG[CT]
BstUI 1632 CGCG CG^CG
BstXI 1035 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1629 GCACGC GC[ACGT]^[ACGT]GC
Cfr10I 1127 ACCGGC [GA]^CCGG[CT]
DraIII 1277 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1281 CCGTGG C^C[GA][CT]GG
Eam1105I 331 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 421 CCTAGG C^C[AT][AT]GG
FbaI 1146 TGATCA T^GATCA
HapII 1128 CCGG C^CGG
HgaI 381 GACGC GACGC(5/10)
Hin1I 381 GACGCC G[GA]^CG[CT]C
HpaII 1128 CCGG C^CGG
MaeII 984 ACGT A^CGT
MamI 898 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1128 CCGG C^CGG
MvnI 1632 CGCG CG^CG
MwoI 1543 GCCAAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 184 GGAACC GG[ACGT]^[ACGT]CC
PleI 1420 GAGTC GAGTC(4/5)
Psp1406I 983 AACGTT AA^CGTT
PstI 716 CTGCAG CTGCA^G
RcaI 57 TCATGA T^CATGA
RsaI 576 GTAC GT^AC
SfaNI 1623 GCATC GCATC(5/9)
SfcI 716 CTGCAG C^T[GA][CT]AG
StyI 421 CCTAGG C^C[AT][AT]GG
TseI 806 GCTGC G^C[AT]GC
XbaI 195 TCTAGA T^CTAGA
XcmI 1538 CCAGTGCCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 54 GGATC GGATC(4/5)
1062 GGATC
AlwNI 938 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1460 CAGTCCCTG
BmyI 27 GAGCCC G[AGT]GC[ACT]^C
1610 GTGCCC
BsaI 1428 GAGACC GGTCTC(1/5)
1591 GAGACC
Bsp1286I 27 GAGCCC G[AGT]GC[ACT]^C
1610 GTGCCC
BssKI 1042 CCTGG ^CC[ACGT]GG
1500 CCTGG
BstNI 1042 CCTGG CC^[AT]GG
1500 CCTGG
Cfr13I 87 GGTCC G^G[ACGT]CC
1635 GGCCC
EcoRII 1042 CCTGG ^CC[AT]GG
1500 CCTGG
Fnu4HI 806 GCTGC GC^[ACGT]GC
1633 GCGGC
HindIII 864 AAGCTT A^AGCTT
1085 AAGCTT
ItaI 806 GCTGC GC^[ACGT]GC
1633 GCGGC
MslI 91 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1230 CACAGATGTG
MvaI 1042 CCTGG CC^[AT]GG
1500 CCTGG
Sau96I 87 GGTCC G^G[ACGT]CC
1635 GGCCC
ScrFI 1042 CCTGG CC^[ACGT]GG
1500 CCTGG
SmlI 763 CTCAAG C^T[CT][GA]AG
824 CTCAAG
StuI 424 AGGCCT AGG^CCT
818 AGGCCT
TaqI 25 TCGA T^CGA
794 TCGA
Tsp45I 391 GTGAC ^GT[GC]AC
1184 GTGAC
TthHB8I 25 TCGA T^CGA
794 TCGA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbsI BfaI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
BsmI BsrDI BsrI BstF5I DdeI
DpnII EarI FokI HaeIII HinfI
HphI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI NdeII NlaIII
NspI Sau3AI TfiI Tru9I Tsp509I
TspRI