Restriction map for
pF1KB6887
##################################
# NO CUT ENZYMES ( 175 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeI
MamI MfeI MluI MroI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
StyI SwaI TfiI Tsp509I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 309 GTGCAC G^TGCAC
ApaLI 309 GTGCAC G^TGCAC
AspI 91 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 69 GGACC G^G[AT]CC
BalI 488 TGGCCA TGG^CCA
BglII 404 AGATCT A^GATCT
BpmI 259 CTGGAG CTGGAG(16/14)
BsmAI 350 GTCTC GTCTC(1/5)
BstF5I 222 CATCC GGATG(2/0)
BstYI 404 AGATCT [GA]^GATC[CT]
DdeI 50 CTCAG C^T[ACGT]AG
DsaI 512 CCGTGG C^C[GA][CT]GG
EaeI 488 TGGCCA [CT]^GGCC[GA]
EarI 16 CTCTTC CTCTTC(1/4)
EcoNI 524 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 222 CATCC GGATG(9/13)
HinfI 159 GACTC G^A[ACGT]TC
HphI 156 GGTGA GGTGA(8/7)
Ksp632I 16 CTCTTC CTCTTC(1/4)
MaeIII 157 GTGAC ^GT[ACGT]AC
MboII 17 TCTTC GAAGA(8/7)
MflI 404 AGATCT [GA]^GATC[CT]
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MseI 395 TTAA T^TAA
NlaIII 430 CATG CATG^
PflFI 91 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 507 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 159 GACTC GAGTC(4/5)
PshAI 451 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 35 CTGCAG CTGCA^G
SacI 436 GAGCTC GAGCT^C
SbfI 34 CCTGCAGG CCTGCA^GG
Sse8387I 34 CCTGCAGG CCTGCA^GG
StuI 334 AGGCCT AGG^CCT
TaqI 125 TCGA T^CGA
Tru9I 395 TTAA T^TAA
Tsp45I 157 GTGAC ^GT[GC]AC
Tth111I 91 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 125 TCGA T^CGA
Van91I 507 CCAATCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 404 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 269 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
531 CAGGAGCTG
AspHI 309 GTGCAC G[AT]GC[AT]^C
436 GAGCTC
BanII 262 GAGCCC G[GA]GC[CT]^C
436 GAGCTC
BsiHKAI 309 GTGCAC G[AT]GC[AT]^C
436 GAGCTC
BsmFI 461 GGGAC GGGAC(10/14)
195 GTCCC
DpnII 123 GATC ^GATC
405 GATC
FauI 72 CCCGC CCCGC(4/6)
314 CCCGC
MaeII 95 ACGT A^CGT
389 ACGT
MboI 123 GATC ^GATC
405 GATC
MspA1I 73 CCGCTG C[AC]G^C[GT]G
495 CAGCGG
NdeII 123 GATC ^GATC
405 GATC
NlaIV 69 GGACCC GG[ACGT]^[ACGT]CC
261 GGAGCC
Sau3AI 123 GATC ^GATC
405 GATC
TspRI 346 CAGTG CA[GC]TG[ACGT][ACGT]^
419 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AfaI AluI BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI RsaI Sau96I ScrFI
SfcI TseI