Restriction map for
pF1KB7615
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AfaI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MseI MspA1I
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 613 GAATTT [GA]^AATT[CT]
AhdI 100 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 672 GGATC GGATC(4/5)
ApaI 346 GGGCCC GGGCC^C
ApoI 613 GAATTT [GA]^AATT[CT]
AspEI 100 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 154 GGATAC GTATCC(6/5)
BcnI 436 CCGGG CC^[GC]GG
BglII 94 AGATCT A^GATCT
BsgI 827 GTGCAG GTGCAG(16/14)
BsiEI 447 CGGCCG CG[GA][CT]^CG
BspMI 465 ACCTGC ACCTGC(4/8)
BsrBI 10 CCGCTC CCGCTC(-3/-3)
BssHII 431 GCGCGC G^CGCGC
BstF5I 716 GGATG GGATG(2/0)
DraIII 299 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 447 CGGCCG [CT]^GGCC[GA]
EagI 447 CGGCCG C^GGCCG
Eam1105I 100 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 447 CGGCCG C^GGCCG
Eco52I 447 CGGCCG C^GGCCG
EcoT14I 87 CCATGG C^C[AT][AT]GG
FokI 716 GGATG GGATG(9/13)
HapII 436 CCGG C^CGG
HincII 547 GTCAAC GT[CT]^[GA]AC
HindII 547 GTCAAC GT[CT]^[GA]AC
HpaII 436 CCGG C^CGG
MslI 877 CACGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 436 CCGG C^CGG
NciI 436 CCGGG CC^[GC]GG
NcoI 87 CCATGG C^CATGG
NdeI 103 CATATG CA^TATG
SacI 118 GAGCTC GAGCT^C
SfaNI 189 GCATC GCATC(5/9)
SfcI 857 CTATAG C^T[GA][CT]AG
SphI 900 GCATGC GCATG^C
StyI 87 CCATGG C^C[AT][AT]GG
TfiI 666 GAATC G^A[AT]TC
XcmI 330 CCAGCCCGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 118 GAGCTC G[AT]GC[AT]^C
381 GTGCTC
AvaI 115 CCCGAG C^[CT]CG[GA]G
761 CTCGGG
AviII 26 TGCGCA TGC^GCA
903 TGCGCA
BanI 200 GGCACC G^G[CT][GA]CC
275 GGCACC
BbsI 868 GAAGAC GAAGAC(2/6)
291 GTCTTC
BpuAI 868 GAAGAC GAAGAC(2/6)
291 GTCTTC
BsiHKAI 118 GAGCTC G[AT]GC[AT]^C
381 GTGCTC
BsmFI 440 GGGAC GGGAC(10/14)
851 GGGAC
BsoBI 115 CCCGAG C^[CT]CG[GA]G
761 CTCGGG
BstAPI 48 GCAAGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
610 GCAGAATTTGC
BstYI 94 AGATCT [GA]^GATC[CT]
672 GGATCT
DpnII 95 GATC ^GATC
673 GATC
DraII 345 AGGGCCC [GA]G^G[ACGT]CC[CT]
346 GGGCCCC
DsaI 41 CCACGG C^C[GA][CT]GG
87 CCATGG
EcoO109I 345 AGGGCCC [GA]G^G[ACGT]CC[CT]
346 GGGCCCC
FspI 26 TGCGCA TGC^GCA
903 TGCGCA
MboI 95 GATC ^GATC
673 GATC
MflI 94 AGATCT [GA]^GATC[CT]
672 GGATCT
NdeII 95 GATC ^GATC
673 GATC
NspI 173 GCATGT [GA]CATG^[CT]
900 GCATGC
Sau3AI 95 GATC ^GATC
673 GATC
XhoII 94 AGATCT [GA]^GATC[CT]
672 GGATCT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AlwNI BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EarI EcoRII FauI Fnu4HI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeIII MboII MnlI MvaI
MvnI MwoI NlaIII NlaIV PleI
Sau96I ScrFI TseI Tsp45I Tsp509I
TspRI