Restriction map for
pF1KB7822
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccI AclI AcsI AflII AflIII
AgeI AhdI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsgI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII KasI MamI MfeI
MluI MluNI MscI MunI NarI
NcoI NdeI NotI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 175 GACGTC GACGT^C
Acc65I 1187 GGTACC G^GTACC
AccIII 74 TCCGGA T^CCGGA
Alw44I 1345 GTGCAC G^TGCAC
AocI 1154 CCTCAGG CC^T[ACGT]AGG
ApaLI 1345 GTGCAC G^TGCAC
Asp718I 1187 GGTACC G^GTACC
BbvCI 1231 GCTGAGG CCTCAGC(-5/-2)
BcgI 1736 GCATATCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 503 CTAG C^TAG
BsaI 492 GGTCTC GGTCTC(1/5)
BseAI 74 TCCGGA T^CCGGA
BsiWI 975 CGTACG C^GTACG
BsmBI 17 CGTCTC CGTCTC(1/5)
BspEI 74 TCCGGA T^CCGGA
BspMI 1224 GCAGGT ACCTGC(4/8)
BsrBI 1264 GAGCGG CCGCTC(-3/-3)
BsrDI 389 GCAATG GCAATG(2/0)
BssHII 1502 GCGCGC G^CGCGC
BstAPI 916 GCACTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1414 TTCGAA TT^CGAA
BstEII 451 GGTGACC G^GT[ACGT]ACC
BstPI 451 GGTGACC G^GT[ACGT]ACC
BstYI 659 AGATCC [GA]^GATC[CT]
Bsu36I 1154 CCTCAGG CC^T[ACGT]AGG
DrdI 511 GACCCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 334 CGGCCG C^GGCCG
EclXI 334 CGGCCG C^GGCCG
Eco52I 334 CGGCCG C^GGCCG
Eco81I 1154 CCTCAGG CC^T[ACGT]AGG
EcoNI 1542 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 451 GGTGACC G^GT[ACGT]ACC
HpaI 1210 GTTAAC GTT^AAC
KpnI 1187 GGTACC GGTAC^C
KspI 904 CCGCGG CCGC^GG
MaeI 503 CTAG C^TAG
MaeII 176 ACGT A^CGT
MflI 659 AGATCC [GA]^GATC[CT]
MroI 74 TCCGGA T^CCGGA
NheI 502 GCTAGC G^CTAGC
NspV 1414 TTCGAA TT^CGAA
PpuMI 798 GGGACCC [GA]G^G[AT]CC[CT]
SacI 202 GAGCTC GAGCT^C
SacII 904 CCGCGG CCGC^GG
SapI 381 GAAGAGC GCTCTTC(1/4)
SfuI 1414 TTCGAA TT^CGAA
SphI 600 GCATGC GCATG^C
TfiI 1661 GAATC G^A[AT]TC
XcmI 1364 CCAGGACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 659 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 905 CGCG CG^CG
1503 CGCG
BglI 208 GCCAAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
608 GCCCAGAGGGC
BsaWI 74 TCCGGA [AT]^CCGG[AT]
1253 TCCGGT
BsiEI 334 CGGCCG CG[GA][CT]^CG
679 CGACCG
BstUI 905 CGCG CG^CG
1503 CGCG
BstXI 578 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAGTGTGCTGG
DraII 780 GGGGCCT [GA]G^G[ACGT]CC[CT]
798 GGGACCC
DsaI 904 CCGCGG C^C[GA][CT]GG
1610 CCACGG
EarI 381 GAAGAG CTCTTC(1/4)
1182 GAAGAG
EcoO109I 780 GGGGCCT [GA]G^G[ACGT]CC[CT]
798 GGGACCC
HincII 667 GTCAAC GT[CT]^[GA]AC
1210 GTTAAC
HindII 667 GTCAAC GT[CT]^[GA]AC
1210 GTTAAC
Ksp632I 381 GAAGAG CTCTTC(1/4)
1182 GAAGAG
MseI 1211 TTAA T^TAA
1258 TTAA
MslI 262 CACCAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
927 CACACAGGTG
MvnI 905 CGCG CG^CG
1503 CGCG
NspI 600 GCATGC [GA]CATG^[CT]
1584 GCATGT
PaeR7I 985 CTCGAG C^TCGAG
1196 CTCGAG
PflMI 1044 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCAGCCAGTGG
PstI 46 CTGCAG CTGCA^G
748 CTGCAG
PvuII 754 CAGCTG CAG^CTG
1579 CAGCTG
SfcI 46 CTGCAG C^T[GA][CT]AG
748 CTGCAG
Tru9I 1211 TTAA T^TAA
1258 TTAA
Van91I 1044 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1670 CCAGCCAGTGG
XhoI 985 CTCGAG C^TCGAG
1196 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI