Restriction map for
pF1KB6878
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I FauI FbaI
FokI FseI HaeII HincII HindII
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MamI MboII MfeI
MluI MluNI MroI MscI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 513 GGCGTC G[GA]^CG[CT]C
AhdI 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 258 GATCC GGATC(4/5)
AspEI 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 592 GAGCAC G[AT]GC[AT]^C
AvaII 468 GGACC G^G[AT]CC
AviII 314 TGCGCA TGC^GCA
BcnI 519 CCCGG CC^[GC]GG
BglII 365 AGATCT A^GATCT
BpmI 388 CTGGAG CTGGAG(16/14)
BsaHI 513 GGCGTC G[GA]^CG[CT]C
BsaWI 338 ACCGGA [AT]^CCGG[AT]
BsiEI 336 CGACCG CG[GA][CT]^CG
BsiHKAI 592 GAGCAC G[AT]GC[AT]^C
BsrFI 211 GCCGGC [GA]^CCGG[CT]
BsrI 527 CCAGT ACTGG(1/-1)
BstAPI 550 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 580 CCTGG CC^[AT]GG
BstXI 567 CCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 365 AGATCT [GA]^GATC[CT]
Cfr10I 211 GCCGGC [GA]^CCGG[CT]
DrdI 135 GACGCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 580 CCTGG ^CC[AT]GG
EcoT22I 181 ATGCAT ATGCA^T
FspI 314 TGCGCA TGC^GCA
Hin1I 513 GGCGTC G[GA]^CG[CT]C
HindIII 452 AAGCTT A^AGCTT
MaeIII 144 GTCAC ^GT[ACGT]AC
MflI 365 AGATCT [GA]^GATC[CT]
MseI 77 TTAA T^TAA
MspA1I 310 CAGCTG C[AC]G^C[GT]G
MvaI 580 CCTGG CC^[AT]GG
NaeI 211 GCCGGC GCC^GGC
NciI 519 CCCGG CC^[GC]GG
NgoMIV 211 GCCGGC G^CCGGC
NsiI 181 ATGCAT ATGCA^T
NspI 183 GCATGT [GA]CATG^[CT]
PvuII 310 CAGCTG CAG^CTG
SfaNI 180 GATGC GCATC(5/9)
TaqI 473 TCGA T^CGA
Tru9I 77 TTAA T^TAA
Tsp45I 144 GTCAC ^GT[GC]AC
Tsp509I 172 AATT ^AATT
TthHB8I 473 TCGA T^CGA
XcmI 561 CCACTCCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 365 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BanII 14 GAGCCC G[GA]GC[CT]^C
523 GAGCCC
BsaJI 518 CCCCGG C^C[ACGT][ACGT]GG
579 CCCTGG
BsmFI 219 GTCCC GGGAC(10/14)
516 GTCCC
BspMI 437 ACCTGC ACCTGC(4/8)
138 GCAGGT
BssKI 519 CCCGG ^CC[ACGT]GG
580 CCTGG
CfoI 300 GCGC GCG^C
315 GCGC
DdeI 155 CTCAG C^T[ACGT]AG
590 CTGAG
HhaI 300 GCGC GCG^C
315 GCGC
HinP1I 300 GCGC G^CGC
315 GCGC
HinfI 217 GAGTC G^A[ACGT]TC
481 GACTC
HphI 160 GGTGA GGTGA(8/7)
98 TCACC
MslI 562 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
568 CATGCTGGTG
PleI 481 GACTC GAGTC(4/5)
217 GAGTC
PstI 43 CTGCAG CTGCA^G
322 CTGCAG
ScrFI 519 CCCGG CC^[ACGT]GG
580 CCTGG
SmlI 244 CTCAAG C^T[CT][GA]AG
414 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BseRI BsiYI BslI BsmAI BsmBI
Bsp1286I Cac8I Cfr13I DpnII Fnu4HI
HaeIII HapII HgaI HpaII ItaI
MboI MnlI MspI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I SfcI
TseI TspRI