Restriction map for
pF1KE0011
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AclI AcsI AflII AflIII
AgeI AhdI AocI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspLU11I BspMI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1753 GACGTC GACGT^C
AccI 384 GTCTAC GT^[AC][GT]AC
AccII 1907 CGCG CG^CG
AccIII 770 TCCGGA T^CCGGA
AcyI 1753 GACGTC G[GA]^CG[CT]C
Alw44I 1936 GTGCAC G^TGCAC
Aor51HI 1281 AGCGCT AGC^GCT
ApaLI 1936 GTGCAC G^TGCAC
AspI 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1763 CTCGGG C^[CT]CG[GA]G
BanI 253 GGCACC G^G[CT][GA]CC
BsaHI 1753 GACGTC G[GA]^CG[CT]C
BseAI 770 TCCGGA T^CCGGA
BsmBI 64 CGTCTC CGTCTC(1/5)
BsoBI 1763 CTCGGG C^[CT]CG[GA]G
BspDI 1735 ATCGAT AT^CGAT
BspEI 770 TCCGGA T^CCGGA
BssSI 316 CACGAG CACGAG(-5/-1)
BstUI 1907 CGCG CG^CG
ClaI 1735 ATCGAT AT^CGAT
Eco47III 1281 AGCGCT AGC^GCT
Hin1I 1753 GACGTC G[GA]^CG[CT]C
HincII 322 GTCAAC GT[CT]^[GA]AC
HindII 322 GTCAAC GT[CT]^[GA]AC
MroI 770 TCCGGA T^CCGGA
MvnI 1907 CGCG CG^CG
NaeI 1543 GCCGGC GCC^GGC
NgoMIV 1543 GCCGGC G^CCGGC
NspI 1247 GCATGC [GA]CATG^[CT]
PflFI 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1776 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 357 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 278 AGTACT AGT^ACT
SphI 1247 GCATGC GCATG^C
Tsp509I 107 AATT ^AATT
Tth111I 954 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1776 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 279 GTAC GT^AC
1302 GTAC
AlwNI 1651 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1777 CAGAGGCTG
BbsI 1470 GAAGAC GAAGAC(2/6)
1642 GAAGAC
BcgI 1266 CGAATCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1727 GCATCCACATCG
BciVI 612 GTATCC GTATCC(6/5)
1018 GTATCC
BfaI 2071 CTAG C^TAG
2395 CTAG
BlpI 885 GCTGAGC GC^T[ACGT]AGC
1899 GCTGAGC
Bpu1102I 885 GCTGAGC GC^T[ACGT]AGC
1899 GCTGAGC
BpuAI 1470 GAAGAC GAAGAC(2/6)
1642 GAAGAC
BsaWI 722 ACCGGA [AT]^CCGG[AT]
770 TCCGGA
BsmI 741 GAATGC GAATGC(1/-1)
1482 GAATGC
BsrBI 757 GAGCGG CCGCTC(-3/-3)
1385 GAGCGG
BsrFI 764 ACCGGC [GA]^CCGG[CT]
1543 GCCGGC
BstAPI 438 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1980 GCAGCGGATGC
BstXI 1022 CCAGCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1205 CCATAACCTTGG
BstYI 1105 GGATCT [GA]^GATC[CT]
1785 GGATCT
CelII 885 GCTGAGC GC^T[ACGT]AGC
1899 GCTGAGC
CfoI 1282 GCGC GCG^C
1342 GCGC
Cfr10I 764 ACCGGC [GA]^CCGG[CT]
1543 GCCGGC
HaeII 1281 AGCGCT [GA]GCGC^[CT]
1341 GGCGCT
HgaI 174 GACGC GACGC(5/10)
212 GCGTC
HhaI 1282 GCGC GCG^C
1342 GCGC
HinP1I 1282 GCGC G^CGC
1342 GCGC
MaeI 2071 CTAG C^TAG
2395 CTAG
MflI 1105 GGATCT [GA]^GATC[CT]
1785 GGATCT
NcoI 389 CCATGG C^CATGG
2339 CCATGG
RsaI 279 GTAC GT^AC
1302 GTAC
SapI 483 GAAGAGC GCTCTTC(1/4)
754 GAAGAGC
SexAI 1931 ACCTGGT A^CC[AT]GGT
2264 ACCTGGT
StuI 1493 AGGCCT AGG^CCT
1925 AGGCCT
TfiI 964 GAATC G^A[AT]TC
1267 GAATC
Tsp45I 419 GTGAC ^GT[GC]AC
557 GTGAC
XcmI 1709 CCATGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2255 CCAACAGCAACCTGG
XhoII 1105 GGATCT [GA]^GATC[CT]
1785 GGATCT
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BalI BanII BbvCI BbvI BcnI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrDI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RcaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI TspRI TthHB8I