Restriction map for
pF1KB6432
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DraII DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AhdI 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 218 GTGCAC G^TGCAC
ApaLI 218 GTGCAC G^TGCAC
AspEI 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcnI 613 CCCGG CC^[GC]GG
BsaAI 628 CACGTA [CT]AC^GT[GA]
BsaBI 559 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 193 GCATTC GAATGC(1/-1)
BspDI 163 ATCGAT AT^CGAT
BstYI 425 AGATCC [GA]^GATC[CT]
ClaI 163 ATCGAT AT^CGAT
DraI 619 TTTAAA TTT^AAA
Eam1105I 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 304 GAAGAG CTCTTC(1/4)
EcoNI 369 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 683 GAATTC G^AATTC
EcoT14I 1010 CCATGG C^C[AT][AT]GG
HaeIII 768 GGCC GG^CC
HapII 614 CCGG C^CGG
HindIII 888 AAGCTT A^AGCTT
HinfI 1006 GAATC G^A[ACGT]TC
HpaII 614 CCGG C^CGG
Ksp632I 304 GAAGAG CTCTTC(1/4)
MamI 559 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 425 AGATCC [GA]^GATC[CT]
MspA1I 33 CAGCGG C[AC]G^C[GT]G
MspI 614 CCGG C^CGG
NciI 613 CCCGG CC^[GC]GG
NcoI 1010 CCATGG C^CATGG
NlaIV 44 GGAACC GG[ACGT]^[ACGT]CC
NspI 713 GCATGC [GA]CATG^[CT]
SfcI 740 CTGTAG C^T[GA][CT]AG
SmlI 832 CTTGAG C^T[CT][GA]AG
SphI 713 GCATGC GCATG^C
StyI 1010 CCATGG C^C[AT][AT]GG
TfiI 1006 GAATC G^A[AT]TC
XbaI 640 TCTAGA T^CTAGA
XhoII 425 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 35 GCGG CCGC(-3/-1)
777 GCGG
AlwI 426 GATCC GGATC(4/5)
436 GATCC
AspHI 85 GTGCTC G[AT]GC[AT]^C
218 GTGCAC
AvaI 27 CCCGAG C^[CT]CG[GA]G
77 CCCGAG
AvaII 661 GGACC G^G[AT]CC
793 GGTCC
BfaI 641 CTAG C^TAG
671 CTAG
BpmI 178 CTCCAG CTGGAG(16/14)
49 CTGGAG
BseRI 982 CTCCTC GAGGAG(10/8)
283 GAGGAG
BsiHKAI 85 GTGCTC G[AT]GC[AT]^C
218 GTGCAC
BsoBI 27 CCCGAG C^[CT]CG[GA]G
77 CCCGAG
BsrDI 206 CATTGC GCAATG(2/0)
548 GCAATG
BsrI 939 ACTGG ACTGG(1/-1)
796 CCAGT
DdeI 136 CTCAG C^T[ACGT]AG
985 CTCAG
DpnII 426 GATC ^GATC
436 GATC
DsaI 11 CCACGG C^C[GA][CT]GG
1010 CCATGG
HphI 37 GGTGA GGTGA(8/7)
150 GGTGA
MaeI 641 CTAG C^TAG
671 CTAG
MaeII 533 ACGT A^CGT
629 ACGT
MboI 426 GATC ^GATC
436 GATC
NdeII 426 GATC ^GATC
436 GATC
NlaIII 714 CATG CATG^
1011 CATG
Sau3AI 426 GATC ^GATC
436 GATC
SspI 760 AATATT AAT^ATT
872 AATATT
TaqI 164 TCGA T^CGA
720 TCGA
Tsp45I 38 GTGAC ^GT[GC]AC
626 GTCAC
TspRI 797 CAGTG CA[GC]TG[ACGT][ACGT]^
938 CACTG
TthHB8I 164 TCGA T^CGA
720 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BbvI
BmyI BsaJI BsiYI BslI BsmAI
Bsp1286I BssKI BstNI Cac8I Cfr13I
EcoRII Fnu4HI ItaI MaeIII MboII
MnlI MseI MvaI MwoI Sau96I
ScrFI SfaNI Tru9I TseI Tsp509I
XmnI