Restriction map for
pF1KE0125
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FseI
FspI HgaI HindIII HinfI HpaI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 334 GTCTAC GT^[AC][GT]AC
AcyI 203 GGCGCC G[GA]^CG[CT]C
AfaI 58 GTAC GT^AC
AlwI 66 GATCC GGATC(4/5)
Aor51HI 107 AGCGCT AGC^GCT
AspI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 203 GGCGCC G^G[CT][GA]CC
BbeI 203 GGCGCC GGCGC^C
BclI 98 TGATCA T^GATCA
BsaHI 203 GGCGCC G[GA]^CG[CT]C
BsaJI 344 CCCAGG C^C[ACGT][ACGT]GG
BsiEI 42 CGACCG CG[GA][CT]^CG
BsmFI 160 GTCCC GGGAC(10/14)
BsrFI 11 ACCGGC [GA]^CCGG[CT]
BstF5I 198 CATCC GGATG(2/0)
BstXI 149 CCAAGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 65 AGATCC [GA]^GATC[CT]
Cfr10I 11 ACCGGC [GA]^CCGG[CT]
DdeI 6 CTCAG C^T[ACGT]AG
Eco47III 107 AGCGCT AGC^GCT
FbaI 98 TGATCA T^GATCA
FokI 198 CATCC GGATG(9/13)
Hin1I 203 GGCGCC G[GA]^CG[CT]C
HincII 172 GTCAAC GT[CT]^[GA]AC
HindII 172 GTCAAC GT[CT]^[GA]AC
KasI 203 GGCGCC G^GCGCC
MboII 236 TCTTC GAAGA(8/7)
MflI 65 AGATCC [GA]^GATC[CT]
NarI 203 GGCGCC GG^CGCC
PflFI 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 211 CTGCAG CTGCA^G
RsaI 58 GTAC GT^AC
SfcI 211 CTGCAG C^T[GA][CT]AG
SfoI 203 GGCGCC GGC^GCC
StuI 230 AGGCCT AGG^CCT
TaqI 104 TCGA T^CGA
Tth111I 166 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 104 TCGA T^CGA
XhoII 65 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 70 CGCG CG^CG
285 CGCG
AvaII 159 GGTCC G^G[AT]CC
165 GGACC
BcnI 93 CCCGG CC^[GC]GG
162 CCCGG
BstNI 138 CCTGG CC^[AT]GG
345 CCAGG
BstUI 70 CGCG CG^CG
285 CGCG
Cac8I 209 GCCTGC GC[ACGT]^[ACGT]GC
228 GCAGGC
CfoI 108 GCGC GCG^C
204 GCGC
Cfr13I 159 GGTCC G^G[ACGT]CC
165 GGACC
EcoRII 138 CCTGG ^CC[AT]GG
345 CCAGG
HaeII 107 AGCGCT [GA]GCGC^[CT]
203 GGCGCC
HaeIII 14 GGCC GG^CC
231 GGCC
HhaI 108 GCGC GCG^C
204 GCGC
HinP1I 108 GCGC G^CGC
204 GCGC
MvaI 138 CCTGG CC^[AT]GG
345 CCAGG
MvnI 70 CGCG CG^CG
285 CGCG
MwoI 15 GCCTTTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
204 GCGCCGCCTGC
NciI 93 CCCGG CC^[GC]GG
162 CCCGG
NlaIV 159 GGTCCC GG[ACGT]^[ACGT]CC
203 GGCGCC
PvuII 127 CAGCTG CAG^CTG
214 CAGCTG
Sau96I 159 GGTCC G^G[ACGT]CC
165 GGACC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BssKI DpnII Fnu4HI HapII HpaII
HphI ItaI MboI MnlI MspA1I
MspI NdeII NlaIII Sau3AI ScrFI
TseI