Restriction map for
pF1KB5675
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII Acc65I AccI AcsI AflII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BclI BfrI BglII BlnI
BsiWI BsmBI Bsp1407I BspDI BspHI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshAI PshBI
PvuI RcaI RsrII SalI SapI
SfiI SfuI SnaBI SpeI SphI
SspBI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 1842 AACGTT AA^CGTT
AflIII 977 ACATGT A^C[GA][CT]GT
AgeI 1165 ACCGGT A^CCGGT
ApaI 2724 GGGCCC GGGCC^C
BbrPI 1366 CACGTG CAC^GTG
BbsI 2043 GAAGAC GAAGAC(2/6)
BbvCI 1209 CCTCAGC CCTCAGC(-5/-2)
BcgI 1316 CGATGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 151 GGATAC GTATCC(6/5)
BfaI 2134 CTAG C^TAG
BglI 319 GCCTTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1506 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1506 GCTCAGC GC^T[ACGT]AGC
BpuAI 2043 GAAGAC GAAGAC(2/6)
BsaBI 2343 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 969 GAATGC GAATGC(1/-1)
BspLU11I 977 ACATGT A^CATGT
CelII 1506 GCTCAGC GC^T[ACGT]AGC
EagI 1183 CGGCCG C^GGCCG
EarI 754 CTCTTC CTCTTC(1/4)
EclXI 1183 CGGCCG C^GGCCG
Eco52I 1183 CGGCCG C^GGCCG
FseI 1184 GGCCGGCC GGCCGG^CC
HincII 2332 GTCAAC GT[CT]^[GA]AC
HindII 2332 GTCAAC GT[CT]^[GA]AC
Ksp632I 754 CTCTTC CTCTTC(1/4)
KspI 746 CCGCGG CCGC^GG
MaeI 2134 CTAG C^TAG
MamI 2343 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1813 TTAA T^TAA
NaeI 1185 GCCGGC GCC^GGC
NgoMIV 1185 GCCGGC G^CCGGC
PinAI 1165 ACCGGT A^CCGGT
PmaCI 1366 CACGTG CAC^GTG
PmlI 1366 CACGTG CAC^GTG
Psp1406I 1842 AACGTT AA^CGTT
SacI 2428 GAGCTC GAGCT^C
SacII 746 CCGCGG CCGC^GG
ScaI 248 AGTACT AGT^ACT
SgrAI 282 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2616 CCCGGG CCC^GGG
SspI 1650 AATATT AAT^ATT
Tru9I 1813 TTAA T^TAA
Tsp509I 1567 AATT ^AATT
XmaCI 2616 CCCGGG C^CCGGG
XmaI 2616 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AocI 1967 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
BsaAI 352 TACGTG [CT]AC^GT[GA]
1366 CACGTG
BsaI 419 GAGACC GGTCTC(1/5)
2857 GAGACC
BseRI 2648 CTCCTC GAGGAG(10/8)
2262 GAGGAG
BsiEI 743 CGACCG CG[GA][CT]^CG
1183 CGGCCG
BsrDI 551 GCAATG GCAATG(2/0)
2240 GCAATG
BssHII 1384 GCGCGC G^CGCGC
1522 GCGCGC
Bsu36I 1967 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
Eco81I 1967 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
EcoNI 1305 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1852 CCTGACACAGG
NcoI 944 CCATGG C^CATGG
2981 CCATGG
PflMI 974 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1362 CCAGCACGTGG
PleI 49 GACTC GAGTC(4/5)
304 GACTC
SbfI 1224 CCTGCAGG CCTGCA^GG
2850 CCTGCAGG
SmlI 2814 CTTGAG C^T[CT][GA]AG
2902 CTCAAG
Sse8387I 1224 CCTGCAGG CCTGCA^GG
2850 CCTGCAGG
StuI 611 AGGCCT AGG^CCT
1964 AGGCCT
Tsp45I 1946 GTGAC ^GT[GC]AC
1958 GTGAC
Van91I 974 CCAACATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1362 CCAGCACGTGG
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# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AspI AvaI AvaII AviII
BalI BanI BanII BbeI BbvI
BcnI BmyI BpmI BsaHI BsaJI
BsaWI BseAI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MluNI MnlI MroI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PflFI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI StyI
TaqI TfiI TseI TspRI Tth111I
TthHB8I XcmI XhoII