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# NO CUT ENZYMES ( 210 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeII MamI MboII
MfeI MflI MluI MroI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 21 enzymes) #
###################################
AciI 237 CCGC CCGC(-3/-1)
AlwI 358 GATCC GGATC(4/5)
AvaII 45 GGTCC G^G[AT]CC
BbvI 306 GCTGC GCAGC(8/12)
BfaI 127 CTAG C^TAG
BpmI 194 CTGGAG CTGGAG(16/14)
BsiYI 48 CCAGGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 48 CCAGGGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 197 GAGAC GTCTC(1/5)
BssSI 173 CACGAG CACGAG(-5/-1)
BstAPI 271 GCAGTTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I 45 GGTCC G^G[ACGT]CC
HinfI 119 GAATC G^A[ACGT]TC
MaeI 127 CTAG C^TAG
MseI 275 TTAA T^TAA
PstI 294 CTGCAG CTGCA^G
Sau96I 45 GGTCC G^G[ACGT]CC
SfaNI 325 GATGC GCATC(5/9)
TfiI 119 GAATC G^A[AT]TC
Tru9I 275 TTAA T^TAA
TseI 306 GCTGC G^C[AT]GC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AluI 177 AGCT AG^CT
333 AGCT
BalI 77 TGGCCA TGG^CCA
146 TGGCCA
BsaJI 48 CCAGGG C^C[ACGT][ACGT]GG
264 CCCAGG
BsrI 134 ACTGG ACTGG(1/-1)
242 ACTGG
BssKI 48 CCAGG ^CC[ACGT]GG
265 CCAGG
BstF5I 105 GGATG GGATG(2/0)
137 GGATG
BstNI 48 CCAGG CC^[AT]GG
265 CCAGG
DpnII 228 GATC ^GATC
358 GATC
EaeI 77 TGGCCA [CT]^GGCC[GA]
146 TGGCCA
EcoRII 48 CCAGG ^CC[AT]GG
265 CCAGG
Fnu4HI 236 GCCGC GC^[ACGT]GC
306 GCTGC
FokI 105 GGATG GGATG(9/13)
137 GGATG
HaeIII 78 GGCC GG^CC
147 GGCC
ItaI 236 GCCGC GC^[ACGT]GC
306 GCTGC
MaeIII 40 GTTAC ^GT[ACGT]AC
164 GTTAC
MboI 228 GATC ^GATC
358 GATC
MluNI 77 TGGCCA TGG^CCA
146 TGGCCA
MnlI 30 CCTC CCTC(7/6)
345 GAGG
MscI 77 TGGCCA TGG^CCA
146 TGGCCA
MvaI 48 CCAGG CC^[AT]GG
265 CCAGG
MwoI 271 GCAGTTAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
376 GCATGGGTGGC
NdeII 228 GATC ^GATC
358 GATC
NlaIII 377 CATG CATG^
386 CATG
Sau3AI 228 GATC ^GATC
358 GATC
ScrFI 48 CCAGG CC^[ACGT]GG
265 CCAGG
TspRI 207 CACTG CA[GC]TG[ACGT][ACGT]^
297 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 1 enzymes) #
#########################################
SfcI