Restriction map for
pF1KB6492
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI MaeI MamI MfeI MluI
MseI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 148 TCCGGA T^CCGGA
AcsI 487 AAATTC [GA]^AATT[CT]
AcyI 3 GGCGCC G[GA]^CG[CT]C
AlwI 1043 GGATC GGATC(4/5)
ApaI 70 GGGCCC GGGCC^C
ApoI 487 AAATTC [GA]^AATT[CT]
AvaI 67 CTCGGG C^[CT]CG[GA]G
BalI 356 TGGCCA TGG^CCA
BbeI 3 GGCGCC GGCGC^C
BbvCI 912 GCTGAGG CCTCAGC(-5/-2)
BsaHI 3 GGCGCC G[GA]^CG[CT]C
BsaI 571 GAGACC GGTCTC(1/5)
BsaWI 148 TCCGGA [AT]^CCGG[AT]
BseAI 148 TCCGGA T^CCGGA
BsgI 649 CTGCAC GTGCAG(16/14)
BsoBI 67 CTCGGG C^[CT]CG[GA]G
BspEI 148 TCCGGA T^CCGGA
BspMI 393 GCAGGT ACCTGC(4/8)
BsrI 504 ACTGG ACTGG(1/-1)
BstAPI 969 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 850 TTCGAA TT^CGAA
BstEII 396 GGTGACC G^GT[ACGT]ACC
BstF5I 438 GGATG GGATG(2/0)
BstPI 396 GGTGACC G^GT[ACGT]ACC
BstXI 992 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1043 GGATCT [GA]^GATC[CT]
EarI 61 CTCTTC CTCTTC(1/4)
EcoNI 975 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 396 GGTGACC G^GT[ACGT]ACC
EcoT14I 992 CCAAGG C^C[AT][AT]GG
FokI 438 GGATG GGATG(9/13)
Hin1I 3 GGCGCC G[GA]^CG[CT]C
HindIII 289 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
Ksp632I 61 CTCTTC CTCTTC(1/4)
KspI 7 CCGCGG CCGC^GG
MaeIII 397 GTGAC ^GT[ACGT]AC
MflI 1043 GGATCT [GA]^GATC[CT]
MluNI 356 TGGCCA TGG^CCA
MroI 148 TCCGGA T^CCGGA
MscI 356 TGGCCA TGG^CCA
NarI 3 GGCGCC GG^CGCC
NlaIII 165 CATG CATG^
NspV 850 TTCGAA TT^CGAA
PflMI 696 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1024 CTGCAG CTGCA^G
SacII 7 CCGCGG CCGC^GG
SapI 60 GCTCTTC GCTCTTC(1/4)
ScaI 101 AGTACT AGT^ACT
SfoI 3 GGCGCC GGC^GCC
SfuI 850 TTCGAA TT^CGAA
SmlI 841 CTCAAG C^T[CT][GA]AG
StyI 992 CCAAGG C^C[AT][AT]GG
Tsp45I 397 GTGAC ^GT[GC]AC
Tsp509I 488 AATT ^AATT
Van91I 696 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 359 CCAAGTATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1043 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 102 GTAC GT^AC
555 GTAC
BanI 3 GGCGCC G^G[CT][GA]CC
418 GGCACC
BbsI 427 GAAGAC GAAGAC(2/6)
474 GAAGAC
BcnI 39 CCCGG CC^[GC]GG
831 CCGGG
BglI 89 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
725 GCCACCAGGGC
BpuAI 427 GAAGAC GAAGAC(2/6)
474 GAAGAC
BsmAI 327 GAGAC GTCTC(1/5)
571 GAGAC
DpnII 244 GATC ^GATC
1044 GATC
DsaI 7 CCGCGG C^C[GA][CT]GG
90 CCACGG
EaeI 227 TGGCCG [CT]^GGCC[GA]
356 TGGCCA
HaeII 3 GGCGCC [GA]GCGC^[CT]
42 GGCGCT
HgaI 385 GACGC GACGC(5/10)
644 GCGTC
HinfI 145 GAGTC G^A[ACGT]TC
329 GACTC
MaeII 560 ACGT A^CGT
605 ACGT
MboI 244 GATC ^GATC
1044 GATC
NciI 39 CCCGG CC^[GC]GG
831 CCGGG
NdeII 244 GATC ^GATC
1044 GATC
PleI 329 GACTC GAGTC(4/5)
145 GAGTC
PpuMI 782 GGGACCT [GA]G^G[AT]CC[CT]
1028 AGGACCT
PvuII 181 CAGCTG CAG^CTG
889 CAGCTG
RsaI 102 GTAC GT^AC
555 GTAC
Sau3AI 244 GATC ^GATC
1044 GATC
SfcI 735 CTACAG C^T[GA][CT]AG
1024 CTGCAG
TspRI 414 CAGTG CA[GC]TG[ACGT][ACGT]^
741 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII AviII BanII BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BssKI BssSI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FspI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NlaIV SacI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I