Restriction map for
pF1KE0523
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# NO CUT ENZYMES ( 182 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaBI
BsaHI BsaI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII CfoI ClaI
CpoI DpnII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MboI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AciI 222 CCGC CCGC(-3/-1)
AcsI 235 AAATTT [GA]^AATT[CT]
AflIII 393 ACGTGT A^C[GA][CT]GT
AgeI 413 ACCGGT A^CCGGT
AocI 214 CCTGAGG CC^T[ACGT]AGG
ApoI 235 AAATTT [GA]^AATT[CT]
Asp700I 667 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 392 CACGTG CAC^GTG
BbsI 256 GAAGAC GAAGAC(2/6)
BpmI 649 CTGGAG CTGGAG(16/14)
BpuAI 256 GAAGAC GAAGAC(2/6)
BsaAI 392 CACGTG [CT]AC^GT[GA]
BsaWI 413 ACCGGT [AT]^CCGG[AT]
BsiYI 289 CCTGTCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 289 CCTGTCCAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 209 GTCCC GGGAC(10/14)
BspMI 173 ACCTGC ACCTGC(4/8)
BsrFI 413 ACCGGT [GA]^CCGG[CT]
Bsu36I 214 CCTGAGG CC^T[ACGT]AGG
Cfr10I 413 ACCGGT [GA]^CCGG[CT]
DraI 231 TTTAAA TTT^AAA
DraII 207 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 158 CCACGG C^C[GA][CT]GG
EarI 667 GAAGAG CTCTTC(1/4)
Eco81I 214 CCTGAGG CC^T[ACGT]AGG
EcoO109I 207 AGGTCCC [GA]G^G[ACGT]CC[CT]
HapII 414 CCGG C^CGG
HpaII 414 CCGG C^CGG
Ksp632I 667 GAAGAG CTCTTC(1/4)
MaeII 393 ACGT A^CGT
MslI 580 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 414 CCGG C^CGG
MwoI 135 GCCTGGCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PinAI 413 ACCGGT A^CCGGT
PleI 654 GAGTC GAGTC(4/5)
PmaCI 392 CACGTG CAC^GTG
PmlI 392 CACGTG CAC^GTG
PpuMI 207 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 175 CTGCAG CTGCA^G
Tsp509I 236 AATT ^AATT
XmnI 667 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 143 AGCT AG^CT
624 AGCT
AvaII 208 GGTCC G^G[AT]CC
219 GGACC
BsmAI 94 GAGAC GTCTC(1/5)
576 GAGAC
Cac8I 41 GCTGGC GC[ACGT]^[ACGT]GC
140 GCAAGC
DraIII 335 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
470 CACGAAGTG
HaeIII 44 GGCC GG^CC
166 GGCC
HgaI 58 GACGC GACGC(5/10)
645 GACGC
HphI 286 TCACC GGTGA(8/7)
526 TCACC
MseI 232 TTAA T^TAA
562 TTAA
NlaIV 154 GGAGCC GG[ACGT]^[ACGT]CC
208 GGTCCC
SfcI 128 CTGTAG C^T[GA][CT]AG
175 CTGCAG
Tru9I 232 TTAA T^TAA
562 TTAA
TspRI 34 CACTG CA[GC]TG[ACGT][ACGT]^
572 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
BbvI BsaJI BssKI BstF5I BstNI
Cfr13I DdeI EcoRII Fnu4HI FokI
HinfI ItaI MaeIII MboII MnlI
MvaI Sau96I ScrFI SfaNI TfiI
TseI Tsp45I