Restriction map for
pF1KB7840
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccIII AclI AcsI AgeI
AhdI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI BalI BamHI
BbsI BcgI BciVI BclI BlpI
Bpu1102I BpuAI BsaBI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HpaI KpnI
MamI MfeI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1583 GACGTC GACGT^C
AccI 709 GTCTAC GT^[AC][GT]AC
AflII 298 CTTAAG C^TTAAG
AflIII 1416 ACGCGT A^C[GA][CT]GT
AocI 1264 CCTGAGG CC^T[ACGT]AGG
Aor51HI 863 AGCGCT AGC^GCT
AspI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 521 TGCGCA TGC^GCA
AvrII 1689 CCTAGG C^CTAGG
BbeI 23 GGCGCC GGCGC^C
BbrPI 657 CACGTG CAC^GTG
BbvCI 796 GCTGAGG CCTCAGC(-5/-2)
BfrI 298 CTTAAG C^TTAAG
BglI 608 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 131 AGATCT A^GATCT
BlnI 1689 CCTAGG C^CTAGG
BsaAI 657 CACGTG [CT]AC^GT[GA]
BsiEI 1398 CGGTCG CG[GA][CT]^CG
BsmI 733 GAATGC GAATGC(1/-1)
BsrBI 27 CCGCTC CCGCTC(-3/-3)
BsrFI 1150 GCCGGC [GA]^CCGG[CT]
BstEII 534 GGTGACC G^GT[ACGT]ACC
BstPI 534 GGTGACC G^GT[ACGT]ACC
BstYI 131 AGATCT [GA]^GATC[CT]
Bsu36I 1264 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1150 GCCGGC [GA]^CCGG[CT]
DraIII 175 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1362 CGGCCA [CT]^GGCC[GA]
EarI 448 GAAGAG CTCTTC(1/4)
Eco47III 863 AGCGCT AGC^GCT
Eco81I 1264 CCTGAGG CC^T[ACGT]AGG
EcoNI 333 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 534 GGTGACC G^GT[ACGT]ACC
FspI 521 TGCGCA TGC^GCA
HindIII 845 AAGCTT A^AGCTT
KasI 23 GGCGCC G^GCGCC
Ksp632I 448 GAAGAG CTCTTC(1/4)
KspI 154 CCGCGG CCGC^GG
MflI 131 AGATCT [GA]^GATC[CT]
MluI 1416 ACGCGT A^CGCGT
MseI 299 TTAA T^TAA
NaeI 1150 GCCGGC GCC^GGC
NarI 23 GGCGCC GG^CGCC
NgoMIV 1150 GCCGGC G^CCGGC
PaeR7I 1088 CTCGAG C^TCGAG
PflFI 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 657 CACGTG CAC^GTG
PmlI 657 CACGTG CAC^GTG
PstI 1095 CTGCAG CTGCA^G
SacI 466 GAGCTC GAGCT^C
SacII 154 CCGCGG CCGC^GG
SfoI 23 GGCGCC GGC^GCC
TaqI 1089 TCGA T^CGA
Tru9I 299 TTAA T^TAA
Tsp509I 320 AATT ^AATT
Tth111I 343 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1089 TCGA T^CGA
XcmI 1310 CCATGCCGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1088 CTCGAG C^TCGAG
XhoII 131 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
ApaI 1037 GGGCCC GGGCC^C
1517 GGGCCC
BfaI 853 CTAG C^TAG
1690 CTAG
BsaI 1216 GAGACC GGTCTC(1/5)
1756 GAGACC
BspMI 1373 ACCTGC ACCTGC(4/8)
309 GCAGGT
BsrDI 142 CATTGC GCAATG(2/0)
1750 GCAATG
DsaI 117 CCACGG C^C[GA][CT]GG
154 CCGCGG
HincII 358 GTCAAC GT[CT]^[GA]AC
676 GTTGAC
HindII 358 GTCAAC GT[CT]^[GA]AC
676 GTTGAC
MaeI 853 CTAG C^TAG
1690 CTAG
NspI 165 GCATGC [GA]CATG^[CT]
996 GCATGC
ScaI 431 AGTACT AGT^ACT
926 AGTACT
SmlI 298 CTTAAG C^T[CT][GA]AG
1088 CTCGAG
SphI 165 GCATGC GCATG^C
996 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TfiI
TseI Tsp45I TspRI