Restriction map for
pF1KE0368
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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BclI
BglI BglII BlnI BsaAI BsaHI
BsaI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstUI
BstZ17I ClaI CpoI DraI Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MfeI MluI MspA1I MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacII SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccIII 455 TCCGGA T^CCGGA
AflII 238 CTTAAG C^TTAAG
AseI 1215 ATTAAT AT^TAAT
AsnI 1215 ATTAAT AT^TAAT
BalI 653 TGGCCA TGG^CCA
BanI 194 GGCACC G^G[CT][GA]CC
BbsI 384 GAAGAC GAAGAC(2/6)
BciVI 1742 GTATCC GTATCC(6/5)
BfrI 238 CTTAAG C^TTAAG
BlpI 1242 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1242 GCTGAGC GC^T[ACGT]AGC
BpuAI 384 GAAGAC GAAGAC(2/6)
BsaBI 874 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 455 TCCGGA [AT]^CCGG[AT]
BseAI 455 TCCGGA T^CCGGA
BsgI 483 CTGCAC GTGCAG(16/14)
BsiEI 29 CGGCCG CG[GA][CT]^CG
BsmBI 165 CGTCTC CGTCTC(1/5)
BspEI 455 TCCGGA T^CCGGA
BsrBI 93 GAGCGG CCGCTC(-3/-3)
BsrDI 549 GCAATG GCAATG(2/0)
BssSI 1037 CTCGTG CACGAG(-5/-1)
BstAPI 736 GCAGGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 962 TTCGAA TT^CGAA
BstEII 1896 GGTCACC G^GT[ACGT]ACC
BstPI 1896 GGTCACC G^GT[ACGT]ACC
BstYI 1827 AGATCC [GA]^GATC[CT]
CelII 1242 GCTGAGC GC^T[ACGT]AGC
DraII 1727 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 211 GACACTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 29 CGGCCG C^GGCCG
EarI 313 GAAGAG CTCTTC(1/4)
EclXI 29 CGGCCG C^GGCCG
Eco52I 29 CGGCCG C^GGCCG
EcoNI 453 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1727 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1896 GGTCACC G^GT[ACGT]ACC
EcoRI 1456 GAATTC G^AATTC
EcoT14I 170 CCTTGG C^C[AT][AT]GG
HgaI 9 GACGC GACGC(5/10)
HincII 401 GTCGAC GT[CT]^[GA]AC
HindII 401 GTCGAC GT[CT]^[GA]AC
HindIII 352 AAGCTT A^AGCTT
Ksp632I 313 GAAGAG CTCTTC(1/4)
MamI 874 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1827 AGATCC [GA]^GATC[CT]
MluNI 653 TGGCCA TGG^CCA
MroI 455 TCCGGA T^CCGGA
MscI 653 TGGCCA TGG^CCA
NaeI 191 GCCGGC GCC^GGC
NgoMIV 191 GCCGGC G^CCGGC
NspV 962 TTCGAA TT^CGAA
PaeR7I 1884 CTCGAG C^TCGAG
PpuMI 1727 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1215 ATTAAT AT^TAAT
SalI 401 GTCGAC G^TCGAC
SfuI 962 TTCGAA TT^CGAA
SmaI 69 CCCGGG CCC^GGG
SspI 1506 AATATT AAT^ATT
StyI 170 CCTTGG C^C[AT][AT]GG
XbaI 1080 TCTAGA T^CTAGA
XcmI 864 CCATGATAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1884 CTCGAG C^TCGAG
XhoII 1827 AGATCC [GA]^GATC[CT]
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 401 GTCGAC GT^[AC][GT]AC
559 GTCTAC
AcsI 1456 GAATTC [GA]^AATT[CT]
1526 AAATTT
AlwI 469 GATCC GGATC(4/5)
1828 GATCC
AlwNI 477 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
1237 CAGATGCTG
AocI 532 CCTGAGG CC^T[ACGT]AGG
778 CCTCAGG
ApoI 1456 GAATTC [GA]^AATT[CT]
1526 AAATTT
BfaI 1081 CTAG C^TAG
1915 CTAG
BseRI 1667 CTCCTC GAGGAG(10/8)
44 GAGGAG
BsmAI 1233 GAGAC GTCTC(1/5)
166 GTCTC
BsmFI 1796 GGGAC GGGAC(10/14)
202 GTCCC
BsmI 856 GCATTC GAATGC(1/-1)
1591 GCATTC
BsrFI 80 GCCGGT [GA]^CCGG[CT]
191 GCCGGC
BstXI 1746 CCAGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCATCATGTTGG
Bsu36I 532 CCTGAGG CC^T[ACGT]AGG
778 CCTCAGG
Cfr10I 80 GCCGGT [GA]^CCGG[CT]
191 GCCGGC
DraIII 507 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
1012 CACACTGTG
DsaI 564 CCGTGG C^C[GA][CT]GG
1421 CCGTGG
EaeI 29 CGGCCG [CT]^GGCC[GA]
653 TGGCCA
Eco81I 532 CCTGAGG CC^T[ACGT]AGG
778 CCTCAGG
HphI 1477 GGTGA GGTGA(8/7)
1898 TCACC
MaeI 1081 CTAG C^TAG
1915 CTAG
MaeII 200 ACGT A^CGT
641 ACGT
MslI 1468 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1703 CATATTTGTG
MwoI 80 GCCGGTGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
736 GCAGGATATGC
PstI 1279 CTGCAG CTGCA^G
1391 CTGCAG
ScaI 1191 AGTACT AGT^ACT
1364 AGTACT
SfcI 1279 CTGCAG C^T[GA][CT]AG
1391 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI NciI NdeII NlaIII NlaIV
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I