Restriction map for
pF1KB8646
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BalI
BanI BbeI BbrPI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI Sse8387I SspBI
SspI SwaI Tru9I Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 15 GACGTC GACGT^C
AcyI 15 GACGTC G[GA]^CG[CT]C
AscI 554 GGCGCGCC GG^CGCGCC
AspHI 704 GAGCAC G[AT]GC[AT]^C
AvaII 321 GGACC G^G[AT]CC
AviII 485 TGCGCA TGC^GCA
BamHI 215 GGATCC G^GATCC
BbsI 570 GAAGAC GAAGAC(2/6)
BbvCI 114 GCTGAGG CCTCAGC(-5/-2)
BfaI 677 CTAG C^TAG
BpmI 169 CTGGAG CTGGAG(16/14)
BpuAI 570 GAAGAC GAAGAC(2/6)
BsaHI 15 GACGTC G[GA]^CG[CT]C
BseRI 249 CTCCTC GAGGAG(10/8)
BsgI 4 GTGCAG GTGCAG(16/14)
BsiHKAI 704 GAGCAC G[AT]GC[AT]^C
BsmBI 47 CGTCTC CGTCTC(1/5)
BsmI 54 GAATGC GAATGC(1/-1)
BspHI 365 TCATGA T^CATGA
BsrBI 538 CCGCTC CCGCTC(-3/-3)
BsrFI 329 GCCGGT [GA]^CCGG[CT]
BssHII 555 GCGCGC G^CGCGC
BstXI 161 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 215 GGATCC [GA]^GATC[CT]
Cfr10I 329 GCCGGT [GA]^CCGG[CT]
DdeI 115 CTGAG C^T[ACGT]AG
EcoNI 581 CCTCTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 38 CCTTGG C^C[AT][AT]GG
FspI 485 TGCGCA TGC^GCA
HgaI 46 GCGTC GACGC(5/10)
Hin1I 15 GACGTC G[GA]^CG[CT]C
MaeI 677 CTAG C^TAG
MaeIII 198 GTTAC ^GT[ACGT]AC
MflI 215 GGATCC [GA]^GATC[CT]
MslI 159 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 622 CAGCTG C[AC]G^C[GT]G
PleI 270 GACTC GAGTC(4/5)
PpuMI 320 GGGACCC [GA]G^G[AT]CC[CT]
PstI 229 CTGCAG CTGCA^G
PvuII 622 CAGCTG CAG^CTG
RcaI 365 TCATGA T^CATGA
SfcI 229 CTGCAG C^T[GA][CT]AG
SpeI 676 ACTAGT A^CTAGT
SphI 546 GCATGC GCATG^C
StuI 377 AGGCCT AGG^CCT
StyI 38 CCTTGG C^C[AT][AT]GG
Tsp509I 255 AATT ^AATT
TspRI 653 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 215 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 216 GATCC GGATC(4/5)
215 GGATC
BanII 224 GGGCTC G[GA]GC[CT]^C
307 GAGCCC
BbvI 6 GCAGC GCAGC(8/12)
43 GCAGC
BsrI 433 ACTGG ACTGG(1/-1)
652 CCAGT
BstF5I 658 CATCC GGATG(2/0)
66 GGATG
Cfr13I 321 GGACC G^G[ACGT]CC
643 GGGCC
DraII 320 GGGACCC [GA]G^G[ACGT]CC[CT]
642 GGGGCCT
EcoO109I 320 GGGACCC [GA]G^G[ACGT]CC[CT]
642 GGGGCCT
FokI 658 CATCC GGATG(9/13)
66 GGATG
HaeII 111 GGCGCT [GA]GCGC^[CT]
684 AGCGCC
MaeII 16 ACGT A^CGT
71 ACGT
MboII 570 GAAGA GAAGA(8/7)
371 TCTTC
Sau96I 321 GGACC G^G[ACGT]CC
643 GGGCC
SmaI 219 CCCGGG CCC^GGG
390 CCCGGG
TaqI 273 TCGA T^CGA
426 TCGA
TfiI 362 GATTC G^A[AT]TC
711 GAATC
TseI 6 GCAGC G^C[AT]GC
43 GCAGC
TthHB8I 273 TCGA T^CGA
426 TCGA
XmaCI 219 CCCGGG C^CCGGG
390 CCCGGG
XmaI 219 CCCGGG C^CCGGG
390 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AluI AvaI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstNI
BstUI Cac8I CfoI DpnII EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI ScrFI SfaNI