Restriction map for
pF1KE0375
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AccIII AclI AflII AflIII AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BamHI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BseAI BsiHKAI BsiWI
BspEI BspLU11I BsrBI BssHII BssSI
BstAPI BstBI BstXI CelII CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HgaI HpaI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 199 GACGTC GACGT^C
AccI 1353 GTATAC GT^[AC][GT]AC
AccII 664 CGCG CG^CG
AgeI 963 ACCGGT A^CCGGT
AocI 1686 CCTGAGG CC^T[ACGT]AGG
ApaI 1075 GGGCCC GGGCC^C
AvaI 605 CTCGGG C^[CT]CG[GA]G
BbeI 160 GGCGCC GGCGC^C
BbrPI 738 CACGTG CAC^GTG
BbsI 202 GTCTTC GAAGAC(2/6)
BbvCI 297 GCTGAGG CCTCAGC(-5/-2)
BglII 1131 AGATCT A^GATCT
BpuAI 202 GTCTTC GAAGAC(2/6)
BsaAI 738 CACGTG [CT]AC^GT[GA]
BsaI 1364 GGTCTC GGTCTC(1/5)
BsaWI 963 ACCGGT [AT]^CCGG[AT]
BsiEI 1092 CGATCG CG[GA][CT]^CG
BsmI 723 GCATTC GAATGC(1/-1)
BsoBI 605 CTCGGG C^[CT]CG[GA]G
Bsp1407I 677 TGTACA T^GTACA
BspDI 415 ATCGAT AT^CGAT
BspHI 905 TCATGA T^CATGA
BspMI 375 GCAGGT ACCTGC(4/8)
BsrGI 677 TGTACA T^GTACA
Bst1107I 1353 GTATAC GTA^TAC
BstEII 959 GGTTACC G^GT[ACGT]ACC
BstPI 959 GGTTACC G^GT[ACGT]ACC
BstUI 664 CGCG CG^CG
BstZ17I 1353 GTATAC GTA^TAC
Bsu36I 1686 CCTGAGG CC^T[ACGT]AGG
ClaI 415 ATCGAT AT^CGAT
Eco81I 1686 CCTGAGG CC^T[ACGT]AGG
EcoNI 675 CCTGTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 959 GGTTACC G^GT[ACGT]ACC
FauI 1067 CCCGC CCCGC(4/6)
HaeII 160 GGCGCC [GA]GCGC^[CT]
HindIII 1544 AAGCTT A^AGCTT
KasI 160 GGCGCC G^GCGCC
KspI 663 CCGCGG CCGC^GG
MvnI 664 CGCG CG^CG
NarI 160 GGCGCC GG^CGCC
NcoI 1162 CCATGG C^CATGG
NspI 1604 ACATGC [GA]CATG^[CT]
PflMI 713 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 963 ACCGGT A^CCGGT
PleI 1197 GACTC GAGTC(4/5)
PmaCI 738 CACGTG CAC^GTG
PmlI 738 CACGTG CAC^GTG
PstI 1583 CTGCAG CTGCA^G
PvuI 1092 CGATCG CGAT^CG
PvuII 478 CAGCTG CAG^CTG
RcaI 905 TCATGA T^CATGA
SacII 663 CCGCGG CCGC^GG
SapI 984 GAAGAGC GCTCTTC(1/4)
SfoI 160 GGCGCC GGC^GCC
SspBI 677 TGTACA T^GTACA
SspI 1475 AATATT AAT^ATT
Van91I 713 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1217 CCATATATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1535 GGTACC G^GTACC
1629 GGTACC
AcyI 160 GGCGCC G[GA]^CG[CT]C
199 GACGTC
Asp718I 1535 GGTACC G^GTACC
1629 GGTACC
BalI 521 TGGCCA TGG^CCA
1126 TGGCCA
BanII 1075 GGGCCC G[GA]GC[CT]^C
1588 GAGCCC
BciVI 1300 GGATAC GTATCC(6/5)
1396 GGATAC
BfaI 188 CTAG C^TAG
826 CTAG
BsaHI 160 GGCGCC G[GA]^CG[CT]C
199 GACGTC
BseRI 1681 CTCCTC GAGGAG(10/8)
1689 GAGGAG
BsgI 307 CTGCAC GTGCAG(16/14)
932 GTGCAG
BsmBI 241 GAGACG CGTCTC(1/5)
631 GAGACG
BsrDI 1494 CATTGC GCAATG(2/0)
610 GCAATG
BstYI 468 GGATCT [GA]^GATC[CT]
1131 AGATCT
Cac8I 749 GCAAGC GC[ACGT]^[ACGT]GC
1578 GCTTGC
DraIII 209 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
735 CACCACGTG
DsaI 663 CCGCGG C^C[GA][CT]GG
1162 CCATGG
EaeI 521 TGGCCA [CT]^GGCC[GA]
1126 TGGCCA
EarI 82 GAAGAG CTCTTC(1/4)
984 GAAGAG
EcoRI 397 GAATTC G^AATTC
1283 GAATTC
EcoT14I 818 CCAAGG C^C[AT][AT]GG
1162 CCATGG
Hin1I 160 GGCGCC G[GA]^CG[CT]C
199 GACGTC
HincII 313 GTCAAC GT[CT]^[GA]AC
1114 GTTGAC
HindII 313 GTCAAC GT[CT]^[GA]AC
1114 GTTGAC
KpnI 1535 GGTACC GGTAC^C
1629 GGTACC
Ksp632I 82 GAAGAG CTCTTC(1/4)
984 GAAGAG
MaeI 188 CTAG C^TAG
826 CTAG
MflI 468 GGATCT [GA]^GATC[CT]
1131 AGATCT
MluNI 521 TGGCCA TGG^CCA
1126 TGGCCA
MscI 521 TGGCCA TGG^CCA
1126 TGGCCA
MslI 1218 CATATATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1605 CATGCAAATG
MspA1I 478 CAGCTG C[AC]G^C[GT]G
663 CCGCGG
PpuMI 73 GGGTCCC [GA]G^G[AT]CC[CT]
821 AGGACCT
StyI 818 CCAAGG C^C[AT][AT]GG
1162 CCATGG
XhoII 468 GGATCT [GA]^GATC[CT]
1131 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI CfoI Cfr10I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I