##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HapII       HhaI        
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        ScrFI       SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI          222  CCGC                 CCGC(-3/-1)
AcyI          236  GACGCC               G[GA]^CG[CT]C
BalI          276  TGGCCA               TGG^CCA
BsaHI         236  GACGCC               G[GA]^CG[CT]C
BseRI          35  CTCCTC               GAGGAG(10/8)
BsiYI         161  CCAGCTCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          161  CCAGCTCTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         142  GTCTC                GTCTC(1/5)
Cfr13I        218  GGCCC                G^G[ACGT]CC
DraII         217  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EaeI          276  TGGCCA               [CT]^GGCC[GA]
EarI          144  CTCTTC               CTCTTC(1/4)
EcoO109I      217  AGGCCCC              [GA]G^G[ACGT]CC[CT]
FauI          221  CCCGC                CCCGC(4/6)
HgaI          236  GACGC                GACGC(5/10)
Hin1I         236  GACGCC               G[GA]^CG[CT]C
Ksp632I       144  CTCTTC               CTCTTC(1/4)
MboII         145  TCTTC                GAAGA(8/7)
MluNI         276  TGGCCA               TGG^CCA
MscI          276  TGGCCA               TGG^CCA
NlaIII        128  CATG                 CATG^
PstI          177  CTGCAG               CTGCA^G
Sau96I        218  GGCCC                G^G[ACGT]CC
SfcI          177  CTGCAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AfaI           47  GTAC                 GT^AC
               71  GTAC                
AlwNI         255  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              297  CAGCTGCTG           
BanII         291  GGGCTC               G[GA]GC[CT]^C
              318  GGGCTC              
BmyI          291  GGGCTC               G[AGT]GC[ACT]^C
              318  GGGCTC              
BsmFI          95  GTCCC                GGGAC(10/14)
              228  GTCCC               
Bsp1286I      291  GGGCTC               G[AGT]GC[ACT]^C
              318  GGGCTC              
HaeIII        218  GGCC                 GG^CC
              277  GGCC                
MspA1I        255  CAGCTG               C[AC]G^C[GT]G
              297  CAGCTG              
PvuII         255  CAGCTG               CAG^CTG
              297  CAGCTG              
RsaI           47  GTAC                 GT^AC
               71  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BbvI        BpmI        BsrI        BstXI       
Cac8I       DdeI        Fnu4HI      ItaI        MnlI        
MwoI        NlaIV       TseI        TspRI