##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   16 enzymes) #
###################################

AvaII         467  GGTCC                G^G[AT]CC
BfaI          827  CTAG                 C^TAG
BsgI          594  GTGCAG               GTGCAG(16/14)
BsmFI         838  GTCCC                GGGAC(10/14)
BstAPI        806  GCAATCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I        467  GGTCC                G^G[ACGT]CC
DpnII         861  GATC                 ^GATC
EarI          621  CTCTTC               CTCTTC(1/4)
Ksp632I       621  CTCTTC               CTCTTC(1/4)
MaeI          827  CTAG                 C^TAG
MboI          861  GATC                 ^GATC
MboII         622  TCTTC                GAAGA(8/7)
NdeII         861  GATC                 ^GATC
SapI          620  GCTCTTC              GCTCTTC(1/4)
Sau3AI        861  GATC                 ^GATC
Sau96I        467  GGTCC                G^G[ACGT]CC


###################################
# TWO CUT ENZYMES (    6 enzymes) #
###################################

AocI          680  CCTCAGG              CC^T[ACGT]AGG
              767  CCTCAGG             
Bsu36I        680  CCTCAGG              CC^T[ACGT]AGG
              767  CCTCAGG             
Cac8I         189  GCCAGC               GC[ACGT]^[ACGT]GC
              719  GCAAGC              
DdeI          681  CTCAG                C^T[ACGT]AG
              768  CTCAG               
Eco81I        680  CCTCAGG              CC^T[ACGT]AGG
              767  CCTCAGG             
TspRI         592  CAGTG                CA[GC]TG[ACGT][ACGT]^
              853  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AluI        AlwNI       BanII       BbvI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BstF5I      EcoT14I     
Fnu4HI      FokI        HapII       HpaII       ItaI        
MnlI        MspA1I      MspI        MwoI        NlaIV       
PvuII       StyI        TseI