##################################
# NO CUT ENZYMES (  199 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        Eam1105I    EarI        
Eco47III    EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AlwI          385  GGATC                GGATC(4/5)
AocI          374  CCTCAGG              CC^T[ACGT]AGG
AspHI         198  GTGCTC               G[AT]GC[AT]^C
BanI          207  GGTGCC               G^G[CT][GA]CC
BglI          101  GCCGTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         372  CTCCTC               GAGGAG(10/8)
BsgI          222  CTGCAC               GTGCAG(16/14)
BsiEI          99  CGGCCG               CG[GA][CT]^CG
BsiHKAI       198  GTGCTC               G[AT]GC[AT]^C
BssKI         203  CCTGG                ^CC[ACGT]GG
BstAPI        413  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         203  CCTGG                CC^[AT]GG
Bsu36I        374  CCTCAGG              CC^T[ACGT]AGG
DdeI          375  CTCAG                C^T[ACGT]AG
DsaI          102  CCGTGG               C^C[GA][CT]GG
EaeI           99  CGGCCG               [CT]^GGCC[GA]
EagI           99  CGGCCG               C^GGCCG
EclXI          99  CGGCCG               C^GGCCG
Eco52I         99  CGGCCG               C^GGCCG
Eco81I        374  CCTCAGG              CC^T[ACGT]AGG
EcoRII        203  CCTGG                ^CC[AT]GG
HaeIII        100  GGCC                 GG^CC
MvaI          203  CCTGG                CC^[AT]GG
PstI          132  CTGCAG               CTGCA^G
ScrFI         203  CCTGG                CC^[ACGT]GG
SfcI          132  CTGCAG               C^T[GA][CT]AG
TspRI         520  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BanII         246  GGGCTC               G[GA]GC[CT]^C
              276  GGGCTC              
BsmFI         445  GTCCC                GGGAC(10/14)
              505  GTCCC               
BstF5I        389  CATCC                GGATG(2/0)
              151  GGATG               
DpnII         386  GATC                 ^GATC
              528  GATC                
FokI          389  CATCC                GGATG(9/13)
              151  GGATG               
HapII          35  CCGG                 C^CGG
               98  CCGG                
HpaII          35  CCGG                 C^CGG
               98  CCGG                
MboI          386  GATC                 ^GATC
              528  GATC                
MnlI          374  CCTC                 CCTC(7/6)
               23  GAGG                
MspI           35  CCGG                 C^CGG
               98  CCGG                
NdeII         386  GATC                 ^GATC
              528  GATC                
Sau3AI        386  GATC                 ^GATC
              528  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        AlwNI       BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
Cac8I       EcoT14I     Fnu4HI      ItaI        MspA1I      
MwoI        NlaIV       PvuII       StyI        TseI